2. Metabolic Enzyme/Protease Cell Cycle/DNA Damage Immunology/Inflammation
  3. Phosphodiesterase (PDE) CDK Interleukin Related
  4. Aristolochic acid D

Aristolochic acid D  (Synonyms: Aristolochic acid-IVa)

Cat. No.: HY-N1465 Purity: 99.74%
COA
SDS
Handling Instructions Technical Support

Aristolochic acid D (Aristolochic acid-IVa) is an orally active PDE2 (IC50: 4.673 μM) and CDK2 (IC50: 25 μM) inhibitor that can be isolated from Aristolochia indica L. Aristolochic acid D exhibits anti-inflammatory activity and is non-carcinogenic and non-nephrotoxic. Aristolochic acid D can be used in the research of inflammation and tumor-related diseases.

For research use only. We do not sell to patients.

Aristolochic acid D

Aristolochic acid D Chemical Structure

CAS No. : 17413-38-6

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Solution
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Solid
1 mg In-stock
5 mg In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Aristolochic acid D:

Aristolochic acid D Related Antibodies

Top Publications Citing Use of Products

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CDK1 CDK2 CDK3 CDK4 CDK5 CDK6 CDK7 CDK8 CDK9 CDK11 CDK12 CDK13 CDK14 CDK16 CDK19 CDC CLK Pho85 CDK10 CDK15 CDK17 CDK18 CDK20 PITSLRE

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IL-1 IL-2 IL-3 IL-4 IL-5 IL-6 IL-8 IL-10 IL-12 IL-13 IL-15 IL-17 IL-23 IL-7 IL-33 IL-22

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  • Purity & Documentation

  • References

  • Customer Review

Description

Aristolochic acid D (Aristolochic acid-IVa) is an orally active PDE2 (IC50: 4.673 μM) and CDK2 (IC50: 25 μM) inhibitor that can be isolated from Aristolochia indica L. Aristolochic acid D exhibits anti-inflammatory activity and is non-carcinogenic and non-nephrotoxic. Aristolochic acid D can be used in the research of inflammation and tumor-related diseases[1][2][3][4][5][6].

IC50 & Target[2][5][6]

IL-6

 

PDE2

4.673 μM (IC50)

CDK2

25 μM (IC50)

Cellular Effect
Cell Line Type Value Description References
Neutrophil IC50
5.78 μg/mL
Compound: 5
Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced superoxide anion generation after 5 mins
Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced superoxide anion generation after 5 mins
[PMID: 21353775]
Neutrophil IC50
8.49 μg/mL
Compound: 5
Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced elastase release after 5 mins
Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced elastase release after 5 mins
[PMID: 21353775]
In Vitro

Aristolochic acid D (0-300 μM; 48 h) exerts no significant cytotoxicity on LLC-PK1 cells and fails to induce caspase-3/7 activation[1].
Aristolochic acid D (50-100 μM; 8 h) significantly reduces the production of TNF-α and IL-6 in RAW 264.7 cells treated with LPS (HY-D1056A1)[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Aristolochic acid D Related Antibodies

ELISA Assay[2]

Cell Line: RAW 264.7 cells treated LPS (HY-D1056A1)
Concentration: 50 and 100 μM
Incubation Time: Pretreated with 2 h, then co-incubation for 6 h
Result: Reduced the production of TNF-α and IL-6.
In Vivo

Aristolochic acid D (400-600 μg/ear; topical application; single dose) exerts an improving effect in the mouse model of ear edema[2].
Aristolochic acid D (100 mg/kg; oral gavage; single dose) significantly reduces TNF-α luciferase activity in TNF-IRES-Luc mice treated with LPS (HY-D1056A1), exhibiting a rapid inhibitory effect on systemic inflammation but with a short duration[2].
Aristolochic acid D (3-10 mg/kg; oral gavage; five times a week; from day 12 to day 42) possesses anti-inflammatory activity in the mouse model of chronic arthritis[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male ICR mice aged 6-8 weeks old treated phorbol 12-myristate 13-acetate (HY-18739) to induce ear edema[2]
Dosage: 400 and 600 μg/ear
Administration: Topical application; single dose
Result: Exhibitd topical anti-inflammatory activity.
Significantly reduced ear weight, inflammatory cell infiltration and edema.
Animal Model: Male BALB/cAnSmoc-Zap70em(W163C)Smoc (SKG) mice aged 6-8 weeks old treated Mannan (HY-W145667) (SKG model that spontaneously develops chronic arthritis closely resembling human Rheumatoid Arthritis)[3]
Dosage: 3 and 10 mg/kg
Administration: Oral gavage; five times a week; from day 12 to day 42
Result: Alleviated mannan-accelerated arthritis symptoms, reducing inflammatory infiltration, improving bone microstructure, decreasing levels of inflammatory factors, and regulating macrophage polarization as well as restoring the Th17/Treg balance.
Molecular Weight

357.27

Formula

C17H11NO8
CAS No.
Appearance

Solid

Color

Yellow to orange

SMILES

O=C(C1=C2C([N+]([O-])=O)=CC3=C(OC)C=C(O)C=C3C2=C(OCO4)C4=C1)O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (69.98 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7990 mL 13.9950 mL 27.9900 mL
5 mM 0.5598 mL 2.7990 mL 5.5980 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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C2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.74%

SDS (644 KB)

COA (255 KB) LCMS (93 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.7990 mL 13.9950 mL 27.9900 mL 69.9751 mL
5 mM 0.5598 mL 2.7990 mL 5.5980 mL 13.9950 mL
10 mM 0.2799 mL 1.3995 mL 2.7990 mL 6.9975 mL
15 mM 0.1866 mL 0.9330 mL 1.8660 mL 4.6650 mL
20 mM 0.1400 mL 0.6998 mL 1.3995 mL 3.4988 mL
25 mM 0.1120 mL 0.5598 mL 1.1196 mL 2.7990 mL
30 mM 0.0933 mL 0.4665 mL 0.9330 mL 2.3325 mL
40 mM 0.0700 mL 0.3499 mL 0.6998 mL 1.7494 mL
50 mM 0.0560 mL 0.2799 mL 0.5598 mL 1.3995 mL
60 mM 0.0467 mL 0.2333 mL 0.4665 mL 1.1663 mL
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Aristolochic acid D Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Aristolochic acid D17413-38-6Aristolochic acid-IVaPhosphodiesterase (PDE)CDKInterleukin RelatedCyclin dependent kinaseILLLC-PK1 cellsRAW 264.7 cellsear edema modelarthritis modelInhibitorinhibitorinhibit

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