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  4. Monoclonal Antibodies Recombinant Antibodies
  5. Wnt2b Antibody (YA2424)

Wnt2b Antibody (YA2424)

Cat. No.: HY-P82679
COA
SDS
User Guide for Antibodies Technical Support

Wnt2b Antibody (YA2424) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2424), targeting Wnt2b, with a predicted molecular weight of 44 kDa (observed band size: 44 kDa). Wnt2b Antibody (YA2424) can be used for WB, ICC/IF experiment in human, mouse, rat background.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

Wnt2b Antibody (YA2424) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2424), targeting Wnt2b, with a predicted molecular weight of 44 kDa (observed band size: 44 kDa). Wnt2b Antibody (YA2424) can be used for WB, ICC/IF experiment in human, mouse, rat background.
Background

The related gene encodes a member of the wingless-type MMTV integration site (WNT) family of highly conserved, secreted signaling factors. WNT family members function in a variety of developmental processes including regulation of cell growth and differentiation and are characterized by a WNT-core domain. The related gene may play a role in human development as well as carcinorelated genesis. Alternative splicing results in multiple transcript variants.
Gene ID
SwissProt ID
Synonyms
WNT13; Wnt2b; XWNT2
Molecular Weight

Predicted band size: 44 kDa; Observed band size: 44 kDa
Purity

Affinity Purified
Conjugation

Non-conjugated
Modification

Unmodified
RRID
Research Field

Stem Cells
Product Categories

Primary Antibody; Recombinant Rabbit Monoclonal Antibody
Clonality

Recombinant, Monoclonal
Host

Rabbit
Reactivity

Human, Mouse, Rat
Dilution Ratio

WB: 1/500-1/1000;IF: 1/50-1/200

  • Western blot analysis was performed on extracts from Hela (lane 1, 20 μg), K562(lane 2, 20 μg), and C6 (lane 3, 20 μg) using Wnt2b Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Mouse/Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
Application

WB, ICC/IF
Appearance

Liquid
Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Isotype

IgG
Sensitivity

Endogenous
Immunogen

A synthetic peptide of human Wnt2b
Database

Entrez Gene: 7482 Human ; 22414 Mouse ; 116466 Rat

SwissProt: Q93097 Human ; O70283 Mouse ;

OMIM: 618168 Human

Documentation
SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Wnt2b Antibody (YA2424) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Wnt2b Antibody (YA2424)
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