ULK3 Antibody (YA4337)

ULK3 Antibody (YA4337) is a mouse-derived and non-conjugated IgG1 antibody, targeting to ULK3. It can be applicated for WB, IHC-P, ICC/IF, FC assays, in the background of human, rat.

ULK3 is a serine/threonine protein kinase that regulates Sonic hedgehog (SHH) signaling and autophagy. In the absence of SHH ligand, ULK3 acts as a negative regulator of SHH signaling by interacting with SUFU, inactivating its kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2, and/or GLI3). In the presence of SHH, ULK3 dissociates from SUFU, undergoes autophosphorylation, and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. In vitro, ULK3 phosphorylates GLI2, as well as GLI1 and GLI3, though less efficiently. Additionally, ULK3 induces autophagy following cellular senescence, functioning as a regulator of autophagy.

Q6PHR2

Predicted band size: 53 kDa; Observed band size: 53 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Mouse

Human, Rat

WB: 1/500-1/2000; IHC: 1/200-1/1000; IF: 1/50-1/200; FC: 1/200-1/400

WB, IHC-P, ICC/IF, FC

Liquid

Supplied in PBS with 0.05% sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Purified recombinant fragment of human ULK3 expressed in E. Coli.