TFIIE alpha Antibody (YA7024)

Cat. No.: HY-P87341: Data Sheet User Guide for Antibodies
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TFIIE alpha Antibody (YA7024) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TFIIE alpha.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

TFIIE alpha Antibody (YA7024) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TFIIE alpha.

Host

Rabbit

Clonality

Recombinant, Monoclonal

Molecular Weight

Predicted band size: 49.5 kDa;

Observed band size: 60 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P29083

Immunogen

Synthetic peptide within Human TFIIE alpha aa 390-439 / 439.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000

Sensitivity	Endogenous	Purity	Protein A affinity purified.
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase

Subcellular Localization：Nucleus

Subunit：Tetramer of two alpha and two beta chains (PubMed:1956398). Interacts with TAF6/TAFII80 (PubMed:7667268). Interacts with ATF7IP (PubMed:19106100). Interacts with SND1 (PubMed:7651391). Part of TBP-based Pol II pre-initiation complex (PIC), in which Pol II core assembles with general transcription factors and other specific initiation factors including GTF2E1, GTF2E2, GTF2F1, GTF2F2, TCEA1, ERCC2, ERCC3, GTF2H2, GTF2H3, GTF2H4, GTF2H5, GTF2A1, GTF2A2, GTF2B and TBP; this large multi-subunit PIC c

Synonyms

FE antibody; General transcription factor IIE 56 kDa subunit antibody; General transcription factor IIE subunit 1 antibody; General transcription factor IIE, polypeptide 1, alpha 56kDa antibody; Gtf2e1 antibody; MAL7P1.86 antibody; T2EA_HUMAN antibody; TF2E1 antibody; TFIIE A antibody; TFIIE alpha antibody

Documentation

User Guide for Antibodies (1077 KB)