SMN1 Antibody (YA5063)

SMN1 Antibody (YA5063) is a mouse-derived and non-conjugated monoclonal antibody, targeting to SMN1. It can be applicated for WB, IHC-P, ICC/IF, ELISA assays, in the background of human, monkey.

The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, playing a crucial role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins (SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, and SNRPG) that assemble into a heptameric protein ring on the Sm site of small nuclear RNA to form the core snRNP (Sm core). In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF, and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A, which regulates core snRNP assembly. The SMN complex accepts the trapped 5Sm proteins from CLNS1A to form an intermediate, with SMN1 acting as a structural backbone alongside GEMIN2 to gather Sm complex subunits. Binding of snRNA within 5Sm triggers the eviction of the SMN complex, allowing SNRPD3 and SNRPB to complete core snRNP assembly. The SMN complex also ensures proper splicing of U12 intron-containing genes, which may be important for normal motor and proprioceptive neuron development. Additionally, it resolves RNA-DNA hybrids formed by RNA polymerase II, which create R-loops in transcription termination regions, a critical step in proper transcription termination. It may also play a role in small nucleolar ribonucleoprotein (snoRNPs) metabolism.

Q16637

Predicted band size: 32 kDa;

affinity purified.

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Monkey

WB: 1:500-1:2000; IHC: 1:200-1:1000; ICC/IF: 1:200-1:1000; ELISA: 1:10000

WB, IHC-P, ICC/IF, ELISA

Liquid

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

Purified recombinant fragment of human SMN1 expressed in E. Coli.