SMC5 Antibody (YA7005)

Cat. No.: HY-P87322: Data Sheet User Guide for Antibodies
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SMC5 Antibody (YA7005) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SMC5.

For research use only. We do not sell to patients.

Size	Price	Stock
10 μL		Ask For Quote & Lead Time
50 μL		Ask For Quote & Lead Time
100 μL		Ask For Quote & Lead Time

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

SMC5 Antibody (YA7005) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SMC5.

Host

Rabbit

Clonality

Recombinant, Monoclonal

Molecular Weight

Predicted band size: 129 kDa;

Observed band size: 129 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat, Monkey

SwissProt ID

Q8IY18

Immunogen

Recombinant protein within human SMC5 aa 901-1,101.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:5000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200

Sensitivity	Endogenous	Purity	Protein A affinity purified.
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Core component of the SMC5-SMC6 complex, a complex involved in repair of DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex

Subcellular Localization：Nucleus; Chromosome; Nucleus, PML body; Chromosome, telomere

Subunit：Forms a heterodimer with SMC6 (PubMed:11408570). Component of the SMC5-SMC6 complex which consists at least of SMC5, SMC6, NSMCE2, NSMCE1, NSMCE4A or EID3 and NSMCE3 (PubMed:18086888). Interacts with NSMCE2 (PubMed:16055714, PubMed:36333305). Interacts with SLF2; this interaction induces an association of the SLF1-SLF2 complex with the SMC5-SMC6 complex (PubMed:25931565, PubMed:36333305). Interacts with RAD18; this interaction is increased in a SLF1 or SLF2-dependent manner (PubMed:25931565)

Synonyms

hSMC 5 antibody; hSMC5 antibody; KIAA0594 antibody; MLZ-453 antibody; OTTHUMP00000063367 antibody; SMC 5 antibody; SMC 5 structural maintenance of chromosomes 5 like 1 antibody; SMC protein 5 antibody; SMC-5 antibody; smc5 antibody

Documentation

User Guide for Antibodies (1077 KB)