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  4. Monoclonal Antibodies Recombinant Antibodies
  5. SMARCB1 Antibody (YA2018)

SMARCB1 Antibody (YA2018)

Cat. No.: HY-P82273
COA
SDS
User Guide for Antibodies Technical Support

SMARCB1 Antibody (YA2018) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SMARCB1.

For research use only. We do not sell to patients.

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10 μL In-stock
50 μL In-stock
100 μL In-stock
250 μL   Get quote  
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Top Publications Citing Use of Products

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

SMARCB1 Antibody (YA2018) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SMARCB1.
Background

The protein encoded by The related gene is part of a complex that relieves repressive chromatin structures, allowing the transcriptional machinery to access its targets more effectively. The encoded nuclear protein may also bind to and enhance the DNA joining activity of HIV-1 integrase. The related gene has been found to be a tumor suppressor, and mutations in it have been associated with malignant rhabdoid tumors. Alternatively spliced transcript variants have been found for The related gene.
Gene ID
SwissProt ID
Synonyms
SMARCB1; BAF47; hSNF5; INI1; RDT; RTPS1; Sfh1p; SMARCB1; SNF5 homolog; SNF5L1; Snr1; SWI/SNF comp
Molecular Weight

Predicted band size: 44 kDa; Observed band size: 44 kDa
Purity

Affinity Purified
Conjugation

Non-conjugated
Modification

Unmodified
Research Field

Epigenetics and Nuclear Signaling
Product Categories

Primary Antibody; Recombinant Rabbit Monoclonal Antibody
Clonality

Recombinant, Monoclonal
Host

Rabbit
Reactivity

Human, Mouse
Dilution Ratio

WB: 1/500-1/1000;IHC: 1/50-1/100;IF: 1/50-1/200;IP: 1/20

  • Western blot analysis was performed on extracts from 293 (lane 1, 15 μg), Hela (lane 2, 15 μg), Jurkat (lane 3, 15 μg), K562 (lane 4, 15 μg), 3T3 (lane 5, 15 μg), and MCF-7 (lane 6, 15 μg) using SMARCB1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
Application

WB, IHC-F, IHC-P, ICC/IF, IP
Appearance

Liquid
Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Isotype

IgG
Sensitivity

Endogenous
Immunogen

Recombinant protein of human SNF5
Database

Entrez Gene: 6598 Human ; 20587 Mouse ; 361825 Rat

SwissProt: Q12824 Human ; Q9Z0H3 Mouse ;

OMIM: 609322 Human

Documentation
SDS (251 KB)

COA (191 KB)

User Guide for Antibodies (1077 KB)

SMARCB1 Antibody (YA2018) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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SMARCB1 Antibody (YA2018)
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