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  5. SEC61A Antibody (YA1825)

SEC61A Antibody (YA1825)

Cat. No.: HY-P82080
COA
SDS
User Guide for Antibodies Technical Support

SEC61A Antibody (YA1825) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SEC61A.

For research use only. We do not sell to patients.

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10 μL In-stock
50 μL In-stock
100 μL In-stock
250 μL   Get quote  
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Top Publications Citing Use of Products

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

SEC61A Antibody (YA1825) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SEC61A.
Background

SEC61A is a component of the SEC61 channel-forming translocon complex that mediates the transport of signal peptide-containing precursor polypeptides across the endoplasmic reticulum (ER). It forms a ribosome receptor and a gated pore in the ER membrane, both functions required for cotranslational translocation of nascent polypeptides.
Gene ID
SwissProt ID
Synonyms
fb62c11; HSEC61; SEC 61; SEC 61A; Sec61 alpha 1; SEC61; SEC61A1
Molecular Weight

Predicted band size: 52 kDa; Observed band size: 48 kDa
Purity

Affinity Chromatography
Conjugation

Non-conjugated
Modification

Unmodified
RRID
Research Field

Signal Transduction
Product Categories

Primary Antibody; Recombinant Rabbit Monoclonal Antibody
Clonality

Recombinant, Monoclonal
Host

Rabbit
Reactivity

Human, Mouse, Rat
Dilution Ratio

WB: 1/500-1/1000;IHC: 1/50-1/100;IF: 1/50-1/200;IP: 1/50;FC: 1/50-1/100

  • Western blot analysis was performed on extracts from Daudi (lane 1, 15 μg), A431 (lane 2, 15 μg), 293 (lane 3, 15 μg), U2OS (lane 4, 15 μg), and Mouse brain (lane 5, 15 μg) using SEC61A Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:2000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:80000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
Application

WB, IHC-P, ICC/IF, IP, FC
Appearance

Liquid
Formulation

Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Isotype

IgG
Sensitivity

Endogenous
Immunogen

A synthesized peptide derived from human SEC61A
Database

Entrez Gene: 29927 Human ; 53421 Mouse ; 80843 Rat

SwissProt: P61619 Human ; P61620 Mouse ; P61621 Rat

OMIM: 617056 Human

Documentation
SDS (251 KB)

COA (191 KB)

User Guide for Antibodies (1077 KB)

SEC61A Antibody (YA1825) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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SEC61A Antibody (YA1825)
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