RING2 Antibody (YA3330)

RING2 Antibody (YA3330) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA3330), targeting RING2, with a predicted molecular weight of 38 kDa (observed band size: 38 kDa). RING2 Antibody (YA3330) can be used for WB, IHC-P experiment in human background.

RING2 is an E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' on histone H2A (H2AK119Ub), playing a central role in the histone code and gene regulation. H2AK119Ub serves as a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation in female mammals (By similarity). It may be involved in initiating both imprinted and random X inactivation (By similarity). As a core component of the Polycomb group (PcG) multiprotein PRC1-like complex, it helps maintain the transcriptionally repressive state of genes, including Hox genes, during development (PubMed:16359901, PubMed:26151332). The PcG PRC1 complex remodels chromatin and modifies histones, leading to heritable changes in chromatin expressibility (PubMed:26151332). Its E3 ubiquitin-protein ligase activity is enhanced by BMI1/PCGF4 (PubMed:21772249). Within the PRC1 complex, RING2 acts as the primary E3 ubiquitin ligase for histone H2A, while RING1 may modulate RNF2/RING2 activity (Probable). Its association with chromosomal DNA is cell-cycle dependent. In resting B- and T-lymphocytes, interaction with AURKB inhibits its activity, sustaining transcription in these cells (By similarity). Additionally, it negatively regulates autophagy by ubiquitinating AMBRA1, leading to its degradation (By similarity).

Q99496

Predicted band size: 38 kDa; Observed band size: 38 kDa

Affinity Purified

Non-conjugated

Unmodified

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1/500-1/1000;IHC: 1/50-1/100

WB, IHC-P

Liquid

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human RING2 / RING1B / RNF2