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  3. Primary Antibodies
  4. Monoclonal Antibodies
  5. BRAF Antibody (YA5221)

BRAF Antibody (YA5221)

Cat. No.: HY-P85529
User Guide for Antibodies Technical Support

BRAF Antibody (YA5221) is a mouse-derived and non-conjugated monoclonal antibody, targeting to Raf-B. It can be applicated for WB assays, in the background of human, mouse.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

BRAF Antibody (YA5221) is a mouse-derived and non-conjugated monoclonal antibody, targeting to Raf-B. It can be applicated for WB assays, in the background of human, mouse.
Background

BRAF is a protein kinase involved in transducing mitogenic signals from the cell membrane to the nucleus (Probable). It phosphorylates MAP2K1, activating the MAP kinase signal transduction pathway (by similar: PubMed:21441910, PubMed:29433126), and can phosphorylate PFKFB2 (by similar: PubMed:36402789). Additionally, it may play a role in the postsynaptic responses of hippocampal neurons (by similar: PubMed:1508179).
Gene ID
SwissProt ID
Synonyms
FLJ95109; 94 kDa B raf protein; B raf 1; B Raf proto oncogene serine threonine protein kinase; B-Raf proto-oncogene serine/threonine-protein kinase; p94; BRAF 1; BRAF; BRAF_HUMAN; BRAF1; cRmil; MGC126806; MGC138284; Murine sarcoma viral; v-raf; oncogene homolog B1; Murine sarcoma viral v raf oncogene homolog B1; NS7; oncogene BRAF1; p94; Proto-oncogene B-Raf; Proto-oncogene c-Rmil; RAFB 1; RAFB1; RMIL; Serine/threonine-protein kinase B-raf; v raf murine sarcoma viral oncogene homolog B; v raf murine sarcoma viral oncogene homolog B1; v-Raf murine sarcoma viral oncogene homolog B1
Molecular Weight

Observed band size: 85 kDa
Purity

affinity chromatography.
Conjugation

Non-conjugated
Modification

Unmodified
Product Categories

Primary Antibody; Mouse Monoclonal Antibody
Clonality

Monoclonal
Host

Mouse
Reactivity

Human, Mouse
Dilution Ratio

WB: 1:1000
Application

WB
Appearance

Liquid
Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Immunogen

Purified recombinant human B Raf protein fragments expressed in E.coli.
Documentation
User Guide for Antibodies (1077 KB)

BRAF Antibody (YA5221) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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BRAF Antibody (YA5221)
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