PRAS40 Antibody (YA6125)

PRAS40 Antibody (YA6125) is a rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PRAS40. It can be applicated for WB, ICC/IF, IP, ELISA assays, in the background of human, mouse, rat.

PRAS40 is a negative regulator of the mechanistic target of rapamycin complex 1 (mTORC1), an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth. In the absence of insulin and nutrients, AKT1S1 associates with the mTORC1 complex and directly inhibits mTORC1 activity by blocking the MTOR substrate-recruitment site. In response to insulin and nutrients, AKT1S1 dissociates from mTORC1. Its activity depends on its phosphorylation state and binding to 14-3-3. Additionally, PRAS40 may play a role in nerve growth factor-mediated neuroprotection (by similar).

Q96B36

Predicted band size: 27 kDa; Observed band size: 40 kDa

Protein A

Non-conjugated

Unmodified

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:2000-1:10000; ICC/IF: 1:200-1:1000; ELISA: 1:5000-1:20000; IP: 1:50-1:200;

WB, ICC/IF, IP, ELISA

Liquid

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG