PARP2 Antibody (YA244)

PARP2 Antibody (YA244) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PARP2.

PARP2 is a poly-ADP-ribosyltransferase that mediates protein poly-ADP-ribosylation and plays a key role in DNA repair. It catalyzes glutamate, aspartate, or serine ADP-ribosylation, transferring the ADP-D-ribosyl group from NAD(+) to target protein carboxyl groups and forming polymers with an average length of 20-30 units. Serine ADP-ribosylation is the primary form in DNA damage response. Without HPF1, PARP2 mediates glutamate and aspartate ADP-ribosylation, while HPF1 binding confers serine specificity. PARP2 recognizes DNA breaks in chromatin, recruits HPF1, and promotes serine ADP-ribosylation of histones, decompacting chromatin and recruiting repair factors. HPF1 restricts PARP2's polymerase activity to limit poly-ADP-ribose chain length. PARP2 also specifically mediates branched poly-ADP-ribosylation, recognized by factors like APLF. Additionally, PARP2 can ADP-ribosylate DNA, preferentially acting on 5'-terminal phosphates at DNA strand breaks in nicked duplexes.

Free

Q9UGN5

Predicted band size: 66 kDa; Observed band size: 66 kDa

Protein A affinity purified.

Chromosome, Nucleus.

Non-conjugated

Unmodified

AB_3102389

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1:500-1:1,000 ;FC: 1:50-1:100

• 
  Western blot analysis of extracts from Hela(lane 2(20μg) or lane 3(40μg)) and Raji(lane 4(20μg) or lane 5(40μg)), using PARP2 (HY-P80265) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80265, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

WB, FC

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human PARP2.AA range:484-583.