NF-KB p100 Antibody (YA4148)

NF-KB p100 Antibody (YA4148) is a mouse-derived and non-conjugated IgG1 antibody, targeting to NFKB2. It can be applicated for WB, ELISA assays, in the background of human, mouse.

NFKB2 is a key member of the NF-kappa-B transcription factor family, existing in two forms, p100 and p52. NF-kappa-B is a pleiotropic transcription factor present in almost all cell types, serving as the endpoint of various signaling pathways involved in inflammation, immunity, differentiation, cell growth, tumorigenesis, and apoptosis. Through the non-canonical activation pathway, p100 associated with RelB is phosphorylated by the MAP3K14-activated CHUK/IKKA homodimer and proteolytically processed into p52, forming the transcriptionally active RelB-p52 heterodimer. Meanwhile, the p52-p52 homodimer acts as a transcriptional repressor. NFKB2 exhibits dual functions: cytoplasmic retention of NF-kappa-B proteins via p100 and generation of p52 through cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 while preserving their independent functions. p52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3' located in the enhancer regions of genes involved in immune response and acute-phase reactions. p100 is the major form, while p52 is the minor form, with relatively inefficient processing of p100. Additionally, the NFKB2 isoform p49 is a subunit of the NF-kappa-B protein complex, synergizing with p65 to stimulate the HIV enhancer. In concert with RELB, it regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-BMAL1 heterodimer.

Q00653

Predicted band size: 97 kDa; Observed band size: 100 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Mouse

Human, Mouse

ELISA: 1/10000

WB, ELISA

Liquid

Supplied in PBS with 0.05% sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Purified recombinant fragment of human NFKB2 (AA: 712-900) expressed in E. Coli.