NEK9 Antibody (YA1362)

NEK9 Antibody (YA1362) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NEK9.

NEK9, a pleiotropic regulator of mitotic progression, orchestrates critical processes in spindle dynamics and chromosome separation. Its pivotal role extends to phosphorylating various substrates, including histones, myelin basic protein, beta-casein, and BICD2. Specifically, NEK9 exhibits phosphorylation activity towards histone H3 on serine and threonine residues and beta-casein on serine residues. The multifaceted influence of NEK9 is underscored by its involvement in the G1/S transition and S phase progression. Moreover, NEK9 plays a key role in modulating the activity of NEK6 and NEK7, enhancing their functionality by releasing the autoinhibitory functions of Tyr-108 and Tyr-97, respectively. This comprehensive regulatory role emphasizes NEK9's significance in governing mitotic processes and ensuring the precise orchestration of cellular events during cell cycle progression.

Q8TD19

Predicted band size: 107 kDa; Observed band size: 107 kDa

Affinity Purified

Non-conjugated

Unmodified

AB_3103727

Cell Biology

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Rat

WB: 1:500-1:1000; IHC: 1:50-1:100; IF: 1:50-1:200

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  Immunocytochemistry analysis of NIH/3T3 cells labeling NEK9 with NEK9 Antibody (HY-P81617) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with NEK9 Antibody (HY-P81617) at 1/50dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of NIH/3T3 cells labeling NEK9 with NEK9 Antibody (HY-P81617) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with NEK9 Antibody (HY-P81617) at 1/100dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

WB, IHC-F, IHC-P, ICC/IF

Liquid

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthetic peptide of human NEK9