2. Antibodies
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  4. Monoclonal Antibodies Recombinant Antibodies
  5. NEK9 Antibody (YA1362)

NEK9 Antibody (YA1362) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA1362), targeting NEK9, with a predicted molecular weight of 107 kDa (observed band size: 107 kDa). NEK9 Antibody (YA1362) can be used for WB, IHC-F, IHC-P, ICC/IF experiment in human, rat background.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

NEK9 Antibody (YA1362) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA1362), targeting NEK9, with a predicted molecular weight of 107 kDa (observed band size: 107 kDa). NEK9 Antibody (YA1362) can be used for WB, IHC-F, IHC-P, ICC/IF experiment in human, rat background.
Background

NEK9, a pleiotropic regulator of mitotic progression, orchestrates critical processes in spindle dynamics and chromosome separation. Its pivotal role extends to phosphorylating various substrates, including histones, myelin basic protein, beta-casein, and BICD2. Specifically, NEK9 exhibits phosphorylation activity towards histone H3 on serine and threonine residues and beta-casein on serine residues. The multifaceted influence of NEK9 is underscored by its involvement in the G1/S transition and S phase progression. Moreover, NEK9 plays a key role in modulating the activity of NEK6 and NEK7, enhancing their functionality by releasing the autoinhibitory functions of Tyr-108 and Tyr-97, respectively. This comprehensive regulatory role emphasizes NEK9's significance in governing mitotic processes and ensuring the precise orchestration of cellular events during cell cycle progression.
Gene ID
SwissProt ID
Synonyms
NC; APUG; NERCC; LCCS10; NERCC1
Molecular Weight

Predicted band size: 107 kDa; Observed band size: 107 kDa
Purity

Affinity Purified
Conjugation

Non-conjugated
Modification

Unmodified
RRID
Research Field

Cell Biology
Product Categories

Primary Antibody; Recombinant Rabbit Monoclonal Antibody
Clonality

Recombinant, Monoclonal
Host

Rabbit
Reactivity

Human, Rat
Dilution Ratio

WB: 1/500-1/1000;IHC: 1/50-1/100;IF: 1/50-1/200
Application

WB, IHC-F, IHC-P, ICC/IF
Appearance

Liquid
Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Isotype

IgG
Sensitivity

Endogenous
Immunogen

A synthetic peptide of human NEK9
Database

Entrez Gene: 91754 Human ; 217718 Mouse ; 299204 Rat

SwissProt: Q8TD19 Human ; Q8K1R7 Mouse ;

OMIM: 617022 Human

Documentation
SDS (251 KB)

COA (191 KB)

User Guide for Antibodies (1077 KB)

NEK9 Antibody (YA1362) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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NEK9 Antibody (YA1362)
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HY-P81617
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