Nanog Antibody (YA4984)

Cat. No.: HY-P85292: Data Sheet User Guide for Antibodies
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Nanog Antibody (YA4984) is a Rabbit-derived and non-conjugated monoclonal antibody, targeting to Nanog.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Nanog Antibody (YA4984) is a Rabbit-derived and non-conjugated monoclonal antibody, targeting to Nanog.

Host

Rabbit

Clonality

Monoclonal, Recombinant

Molecular Weight

Predicted band size: 34 kDa;

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q9H9S0

Gene ID

79923 [NCBI]

Immunogen

Recombinant human Nanog protein (1-100aa). The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-800
IHC-F IHC-F: Immunohistochemistry-Frozen	1:50-800
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-200
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-800

Sensitivity	Endogenous	Purity	affinity purified by Protein A
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repr

Subcellular Localization：Nucleus

Synonyms

NANOG; Homeobox protein NANOG; Homeobox transcription factor Nanog; hNanog

Documentation

User Guide for Antibodies (1077 KB)