Myogenin Antibody (YA5691)

Myogenin Antibody (YA5691) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Myogenin.

Myogenin is a transcriptional activator that promotes the transcription of muscle-specific target genes and plays roles in muscle differentiation, cell cycle exit, and muscle atrophy. It is essential for the development of functional embryonic skeletal muscle fibers but dispensable for postnatal skeletal muscle growth. Phosphorylation by CAMK2G inhibits its transcriptional activity in response to muscle activity. Myogenin is required for recruiting the FACT complex to muscle-specific promoter regions to initiate gene expression. During terminal myoblast differentiation, it acts as a strong transcriptional activator at loci with open chromatin structures previously initiated by MYOD1. It co-occupies muscle-specific gene promoter core regions with MYF5 and MYOD1 during myogenesis and cooperates with the myocyte-specific enhancer factor MEF2D and BRG1-dependent recruitment of SWI/SNF chromatin-remodeling enzymes to alter chromatin structure at myogenic late gene promoters. By upregulating miR-20a expression, it facilitates cell cycle exit during terminal muscle differentiation, where miR-20a represses cell cycle progression genes. It binds to the E-box-containing (E1) promoter region of the miR-20a gene and prevents the reversal of muscle cell differentiation. Additionally, it contributes to atrophy-related gene expression in adult denervated muscles. By similarity, it also induces fibroblast differentiation into myoblasts.

P15173

Predicted band size: 25 kDa;

affinity chromatography.

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Mouse, Rat

IHC: 1:50-200; ICC/IF: 1:50-200; ELISA: 1:500-5000

IHC-P, ICC/IF, ELISA

Liquid

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Synthesized peptide derived from human Myogenin AA range: 100-200