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  5. Myosin Heavy Chain Smooth Muscle Antibody (YA1620)

Myosin Heavy Chain Smooth Muscle Antibody (YA1620)

Cat. No.: HY-P81875
COA
SDS
User Guide for Antibodies Technical Support

Myosin Heavy Chain Smooth Muscle Antibody (YA1620) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Myosin Heavy Chain Smooth Muscle.

For research use only. We do not sell to patients.

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50 μL In-stock
100 μL In-stock
250 μL   Get quote  
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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

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Description

Myosin Heavy Chain Smooth Muscle Antibody (YA1620) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Myosin Heavy Chain Smooth Muscle.
Host

Rabbit
Clonality

Recombinant, Monoclonal
Molecular Weight
Predicted band size: 227 kDa;
Observed band size: 250 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse
SwissProt ID
Gene ID
Immunogen

A synthesized peptide derived from human Myosin, smooth muscle heavy chain 1 and 2 aa2-34.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:50-1:100
Sensitivity Endogenous Purity Affinity Chromatography
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Verification Image
WB

  • Western blot analysis was performed on extracts from Mouse lung (lane 1, 15 μg) using smooth muscle Myosin heavy chain 11 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:40000 dilution) was then applied for 40 minutes at 37°C.

Background
Function：Muscle contraction
Subcellular Localization：Melanosome
Expression：
Tissue_specificity:Smooth muscle; expressed in the umbilical artery, bladder, esophagus, and trachea. Isotype 1 is mainly found in slowly contracting tonifying muscles.
Subunit：Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2)
Database

Entrez Gene: 4629 Human ;

SwissProt: P35749 Human ; O08638 Mouse ; Q63862 Rat

OMIM: 132900 Human

Research Field

Signal Transduction
Synonyms
AAT4; FAA4; MYH11; Myosin 11; Myosin heavy chain 11; Myosin heavy chain smooth muscle isoform; Myosin heavy polypeptide 11 smooth muscle; SMHC; SMMHC
Documentation
SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Myosin Heavy Chain Smooth Muscle Antibody (YA1620) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Myosin Heavy Chain Smooth Muscle Antibody (YA1620)
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