MSH2 Antibody (YA4019)

MSH2 Antibody (YA4019) is a mouse-derived and non-conjugated IgG1 antibody, targeting to MSH2. It can be applicated for WB, IHC-P, ICC/IF, ELISA, ELISA assays, in the background of human, mouse.

MSH2 protein is a key component of the post-replicative DNA mismatch repair system (MMR), crucial for maintaining genomic stability. Forming two distinct heterodimers, MutS alpha (MSH2-MSH6) and MutS beta (MSH2-MSH3), MSH2 recognizes and binds to DNA mismatches, initiating repair processes. MutS alpha targets single base mismatches and dinucleotide insertion-deletion loops (IDL), while MutS beta recognizes larger insertion-deletion loops. Upon mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, directing downstream MMR events such as strand discrimination, excision, and resynthesis. MSH2's ATPase activity is crucial for mismatch repair, acting as a molecular switch that undergoes ADP-->ATP exchange upon mismatch recognition, enabling its conversion into a sliding clamp for hydrolysis-independent diffusion along DNA. Additionally, MSH2 may participate in DNA homologous recombination repair and modulate UV-B-induced cell cycle regulation and apoptosis in melanocytes. As part of the DNA MMR complex, MSH2 collaborates with MSH3, MSH6, PMS1, and MLH1, forming a highly coordinated system essential for genome integrity. MSH2 engages in various protein interactions, including those with MCM9, MCM8, EXO1, ATR, SLX4/BTBD12, and SMARCAD1, highlighting its multifaceted role in maintaining DNA fidelity and participating in broader cellular processes.

P43246

Predicted band size: 105 kDa; Observed band size: 105 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Mouse

Human, Mouse

WB: 1/500-1/2000; IHC: 1/100-1/500; IF: 1/50-1/500; ELISA: 1/10000

WB, IHC-P, ICC/IF, ELISA, ELISA

Liquid

Supplied in PBS with 0.05% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Purified recombinant fragment of human MSH2 expressed in E. Coli.