MAPKAPK5 Antibody (YA5025)

MAPKAPK5 Antibody (YA5025) is a mouse-derived and non-conjugated monoclonal antibody, targeting to MAPKAPK5. It can be applicated for WB, IHC-P, ICC/IF, ELISA assays, in the background of human.

MAPKAPK5 is a tumor suppressor serine/threonine-protein kinase involved in mTORC1 signaling and post-transcriptional regulation. It phosphorylates FOXO3, ERK3/MAPK6, ERK4/MAPK4, HSP27/HSPB1, p53/TP53, and RHEB. Acting as a tumor suppressor, MAPKAPK5 mediates Ras-induced senescence and phosphorylates p53/TP53. It also regulates MYC post-transcriptionally by phosphorylating FOXO3, which promotes FOXO3 nuclear localization and subsequent expression of miR-34b and miR-34c, two miRNAs that bind to the 3'UTR of MYC transcript and inhibit its translation. Additionally, MAPKAPK5 negatively regulates mTORC1 signaling by phosphorylating and inhibiting RHEB. Through its interaction with ERK3/MAPK6 or ERK4/MAPK4, MAPKAPK5 participates in atypical MAPK signaling: the exact role of the complex remains unclear, but it involves a cascade of phosphorylation events where ERK3/MAPK6 (or ERK4/MAPK4) is phosphorylated upon interaction, leading to MAPKAPK5 activation, which in turn phosphorylates ERK3/MAPK6 (or ERK4/MAPK4). In response to PKA/PRKACA stimulation, MAPKAPK5 phosphorylates HSP27/HSPB1, inducing F-actin rearrangement.

Q8IW41

Predicted band size: 54 kDa;

affinity purified.

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human

WB: 1:500-1:2000; IHC: 1:200-1:1000; ELISA: 1:10000; ICC/IF: 1:50-200

WB, IHC-P, ICC/IF, ELISA

Liquid

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

Purified recombinant fragment of PRAK expressed in E. Coli.