LEF1 Antibody (YA3164)

LEF1 Antibody (YA3164) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to LEF1.

LEF1 is a transcription factor that binds DNA in a sequence-specific manner. It participates in the Wnt signaling pathway (By similarity) and activates transcription of target genes in the presence of CTNNB1 and EP300 (By similarity). PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). TLE1, TLE2, TLE3, and TLE4 repress transactivation mediated by LEF1 and CTNNB1. LEF1 regulates T-cell receptor alpha enhancer function and promotes IL17A-expressing gamma-delta T-cell maturation and development by binding to regulatory loci of BLK to modulate its expression (By similarity). During mesenchymal tooth germ formation, LEF1 acts as a positive regulator of odontoblast differentiation, but its expression is repressed during the bell stage by MSX1-mediated inhibition of CTNNB1 signaling (By similarity). LEF1 may also play a role in hair cell differentiation and follicle morphogenesis (By similarity). Additionally, LEF1 transcriptionally activates MYC and CCND1 expression, enhancing the proliferation of pancreatic tumor cells. Some LEF1 isoforms lack the CTNNB1 interaction domain and may act as antagonists of Wnt signaling. LEF1 also transcriptionally activates the fibronectin promoter and binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, contributing to reduced cellular aggregation and increased migration of pancreatic cancer cells.

Q9UJU2

Predicted band size: 44 kDa; Observed band size: 25-58 kDa

Affinity Purified

Non-conjugated

Unmodified

AB_3105530

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1:500-1:1000; IF: 1:50-1:200

• 
  Western blot analysis of extracts from Jurkat (lane 2(20μg) and Jurkat (lane 3(40μg) using LEF1 (HY-P83419) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of HT-29 cells labeling LEF1 Antibody (HY-P83419) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with LEF1 Antibody (HY-P83419) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of Jurkat cells labeling LEF1 Antibody (HY-P83419) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with LEF1 Antibody (HY-P83419) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

WB, ICC/IF

Liquid

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthetic peptide of human LEF1