IKKα Antibody (YA6076)

IKKα Antibody (YA6076) is a rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IKKα. It can be applicated for WB, IHC-P, ICC/IF, IP, ELISA assays, in the background of human, mouse, rat.

CHUK is a serine kinase that plays a pivotal role in the NF-kappa-B signaling pathway, which is activated by various stimuli including inflammatory cytokines, bacterial or viral products, and DNA damage. As part of the canonical IKK complex, CHUK phosphorylates NF-kappa-B inhibitors on serine residues, leading to their ubiquitination and subsequent proteasomal degradation, thereby releasing NF-kappa-B to translocate into the nucleus and activate the transcription of hundreds of genes involved in immune response, growth control, or anti-apoptosis. Additionally, CHUK negatively regulates the pathway by phosphorylating the scaffold protein TAXBP1 to promote the assembly of the A20/TNFAIP3 ubiquitin-editing complex, limiting inflammatory gene activation. In the non-canonical NF-kappa-B pathway, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes that regulate B-cell survival and lymphoid organogenesis. CHUK also participates in the negative feedback of this pathway by phosphorylating and destabilizing MAP3K14/NIK. Within the nucleus, CHUK phosphorylates CREBBP to enhance its transcriptional and histone acetyltransferase activities, and modulates chromatin accessibility at NF-kappa-B-responsive promoters by phosphorylating histone H3 at Ser-10, which is subsequently acetylated at Lys-14 by CREBBP. Furthermore, CHUK phosphorylates the CREBBP-interacting protein NCOA3 and the pro-apoptotic transcription factor FOXO3. By similarity, CHUK phosphorylates RIPK1 at Ser-25 to repress its kinase activity, preventing TNF-mediated RIPK1-dependent cell death. Following mitophagy induction, CHUK phosphorylates AMBRA1 to promote its interaction with ATG8 family proteins and enhance its mitophagic activity.

O15111

Predicted band size: 85 kDa; Observed band size: 85 kDa

Protein A

Non-conjugated

Unmodified

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Monoclonal

Rabbit

Human, Mouse, Rat

IHC: 1:100-1:500; WB: 1:2000-1:10000; ICC/IF: 1:200-1:1000; ELISA: 1:5000-1:20000; IP: 1:50-1:200;

WB, IHC-P, ICC/IF, IP, ELISA

Liquid

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG