Hsp90 alpha/beta Antibody (YA356)

Hsp90 alpha/beta Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 85 kDa, targeting to Hsp90 alpha/beta. It can be used for WB,IHC-F,IHC-P,ICC/IF,IP assays with tag free, in the background of Human, Mouse, Rat.

HSP90AA1 protein, a molecular chaperone, plays a crucial role in the maturation, structural maintenance, and regulation of specific target proteins involved in cell cycle control and signal transduction. Operating through a functional cycle linked to its ATPase activity, essential for its chaperone function, HSP90AA1 induces conformational changes in client proteins, activating them. Dynamic interactions with co-chaperones modulate substrate recognition, the ATPase cycle, and chaperone function. Engaging with diverse client protein classes through co-chaperones, it forms functional chaperones that release properly folded client proteins and co-chaperones in an ADP-bound partially open conformation. Apart from its chaperone activity, HSP90AA1 plays a critical role in mitochondrial import by delivering preproteins to the mitochondrial import receptor TOMM70. It also contributes to the regulation of the transcription machinery at multiple levels, altering transcription factor levels, modulating epigenetic modifiers, and participating in histone eviction from gene promoters. Furthermore, HSP90AA1 binds bacterial lipopolysaccharide, mediating LPS-induced inflammatory responses, including TNF secretion. It antagonizes STUB1-mediated inhibition of TGF-beta signaling and facilitates the association of TOMM70 with IRF3 or TBK1 in the mitochondrial outer membrane, promoting host antiviral responses. In the context of microbial infection, HSP90AA1 interferes with N.meningitidis NadA-mediated invasion of human cells, influencing the adhesion and entry of E.coli expressing NadA into human Chang cells based on its expression levels.

Free

P07900 / P08238

Predicted band size: 85 kDa; Observed band size: 90 kDa

affinity purified

Non-conjugated

Unmodified

AB_3102170

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-1:1,000 ;IHC: 1:50-1:100 ;IF: 1:50-1:200 ;IP: 1:20

WB, IHC-F, IHC-P, ICC/IF, IP

Liquid

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human Hsp90 beta.The exact sequence is proprietary to MCE.