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  4. HLA-DPB1 Antibody (YA3642)

HLA-DPB1 Antibody (YA3642)

Cat. No.: HY-P83945
User Guide for Antibodies Technical Support

HLA-DPB1 Antibody (YA3642) is a mouse-derived and non-conjugated IgG1 antibody, targeting to HLA-DPB1. It can be applicated for IHC-P, FC, ELISA assays, in the background of human, rat.

For research use only. We do not sell to patients.

Size Price Stock Quantity
10 μL Get quote 3 - 4 weeks 2 - 3 weeks 2 - 3 weeks
50 μL Get quote 3 - 4 weeks 2 - 3 weeks 2 - 3 weeks
100 μL Get quote 3 - 4 weeks 2 - 3 weeks 2 - 3 weeks
250 μL   Get quote  
Synthetic products have potential research and development risk.

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Top Publications Citing Use of Products

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

HLA-DPB1 Antibody (YA3642) is a mouse-derived and non-conjugated IgG1 antibody, targeting to HLA-DPB1. It can be applicated for IHC-P, FC, ELISA assays, in the background of human, rat.

Background

The HLA-DPB1 Protein plays a pivotal role in the immune system by binding peptides derived from antigens within the endocytic route of antigen-presenting cells (APCs) and presenting them on the cell surface for recognition by CD4 T-cells. The peptide binding cleft of HLA-DPB1 accommodates peptides ranging from 10 to 30 residues, predominantly generated through the degradation of proteins accessing the endocytic route. This exogenous antigen presentation pathway involves lysosomal proteases and other hydrolases processing antigens taken up by APCs. Notably, cells of the gastrointestinal tract, including epithelial cells, express MHC class II molecules and CD74, acting as unconventional APCs. The assembly of a functional MHC class II molecule involves the association of three MHC class II molecules with a CD74 trimer in the endoplasmic reticulum (ER), forming a heterononamer. Upon entering the endosomal/lysosomal system, CD74 undergoes sequential degradation, leaving a fragment known as CLIP on each MHC class II molecule. HLA-DM facilitates CLIP removal, stabilizing MHC class II until high-affinity antigenic peptides bind. HLA-DO regulates the interaction between HLA-DM and MHC class II in B-cells, and lysosomal acidification influences efficient peptide loading. The MHC class II molecule, bound to a peptide, is then transported to the cell membrane surface for immune recognition.

Gene ID
SwissProt ID
Synonyms
DPB1; HLA-DP; HLA-DPB; HLA-DP1B
Molecular Weight

Predicted band size: 29 kDa; Observed band size: 29 kDa

Purity

affinity purified.

Conjugation

Non-conjugated

Product Categories

Primary Antibody; Mouse Monoclonal Antibody

Host

Mouse

Reactivity

Human, Rat

Dilution Ratio

IHC: 1/200-1/1000; FC: 1/200-1/400; ELISA: 1/10000

Application

IHC-P, FC, ELISA

Appearance

Liquid

Formulation

Supplied in PBS with 0.05% sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Isotype

IgG1

Immunogen

Purified recombinant fragment of human HLA-DPB1 (AA: extra 30-215) expressed in E. Coli.

Documentation

HLA-DPB1 Antibody (YA3642) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
HLA-DPB1 Antibody (YA3642)
Cat. No.:
HY-P83945
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