Hck Antibody (YA4889)

Hck Antibody (YA4889) is a mouse-derived and non-conjugated monoclonal antibody, targeting to Hck. It can be applicated for WB, IHC-P, ICC/IF, ELISA assays, in the background of human.

HCK Protein, a non-receptor tyrosine-protein kinase predominantly found in hematopoietic cells, serves as a pivotal mediator transmitting signals from various cell surface receptors and playing a crucial role in the regulation of innate immune responses. It intricately modulates functions in neutrophils, monocytes, macrophages, and mast cells, influencing processes such as phagocytosis, cell survival, proliferation, adhesion, and migration. Acting downstream of receptors including FCGR1A, FCGR2A, CSF3R, PLAUR, IFNG, IL2, IL6, IL8, and integrins ITGB1 and ITGB2, HCK orchestrates critical events during the phagocytic process, including mobilization of secretory lysosomes, degranulation, and activation of NADPH oxidase, leading to the respiratory burst and release of inflammatory molecules. Additionally, HCK promotes the reorganization of the actin cytoskeleton, actin polymerization, formation of podosomes, and cell protrusions. It inhibits TP73-mediated transcription activation and apoptosis while phosphorylating key signaling proteins such as CBL, ADAM15, BCR, ELMO1, FCGR2A, GAB1, GAB2, RAPGEF1, STAT5B, TP73, VAV1, and WAS, highlighting its extensive involvement in diverse cellular pathways.

P08631

Predicted band size: 60 kDa;

affinity purified.

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human

WB: 1:500-1:2000; IHC: 1:200-1:1000; ELISA: 1:10000; ICC/IF: 1:50-200

WB, IHC-P, ICC/IF, ELISA

Liquid

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

Purified recombinant fragment of Hck expressed in E. Coli.