Hamartin Antibody (YA743)

Hamartin Antibody (YA743) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Hamartin.

Hamartin is a non-catalytic component of the TSC-TBC complex, which acts as a negative regulator of the canonical mTORC1 complex—an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth. The TSC-TBC complex functions as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of mTORC1's protein kinase activity. Under nutrient deprivation, the TSC-TBC complex inhibits mTORC1, preventing phosphorylation of ribosomal protein S6 kinase (RPS6KB1 and RPS6KB2) and EIF4EBP1 (4E-BP1). Nutrient availability inactivates the TSC-TBC complex, relieving mTORC1 inhibition. Within the complex, TSC1 stabilizes TSC2 and prevents its self-aggregation. Hamartin also acts as a tumor suppressor (By similarity: involved in microtubule-mediated protein transport via mTORC1 signaling regulation). Additionally, it serves as a co-chaperone for HSP90AA1, facilitating the chaperoning of client proteins such as kinases, TSC2, and glucocorticoid receptor NR3C1. It enhances ATP binding to HSP90AA1 while inhibiting its ATPase activity. By competing with the activating co-chaperone AHSA1 for HSP90AA1 binding, it provides reciprocal regulation for client protein chaperoning. Furthermore, it recruits TSC2 to HSP90AA1 and stabilizes TSC2 by blocking its interaction with the ubiquitin ligase HERC1.

Free

Q92574

Predicted band size: 130 kDa; Observed band size: 140 kDa

Protein G affinity purified.

Cytoplasm, Membrane.

Non-conjugated

Unmodified

AB_3102571

Cell Biology

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Mouse

WB: 1:500; ICC: 1:100; IHC-P: 1:200-1:600; FC: 1:500-1:1000

• 
  Western blot analysis of extracts from Hela (lane 1) and NIH3T3 (lane 2) using Hamartin antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

WB, ICC/IF, IHC-P, FC

Liquid

Supplied in 1*PBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Endogenous

Synthetic peptide corresponding to Human Hamartin.AA range:401-600.