GPER1 Antibody (YA5888)

GPER1 Antibody (YA5888) is a rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GPER1. It can be applicated for WB, IHC-P, ICC/IF, IP, ELISA assays, in the background of human, mouse, rat.

GPER1 is a G-protein coupled estrogen receptor that binds 17-beta-estradiol (E2) with high affinity, triggering rapid transient activation of multiple intracellular signaling pathways. It stimulates cAMP production, calcium mobilization, and Src tyrosine kinase activity, inducing heparin-bound epidermal growth factor (HB-EGF) release and subsequent EGFR transactivation to initiate downstream pathways including PI3K/Akt and ERK/MAPK. This receptor mediates pleiotropic functions across cardiovascular, endocrine, reproductive, immune, and central nervous systems: providing cardioprotection by reducing hypertrophy and perivascular fibrosis via RAMP3; regulating blood pressure through vasodilation and inhibition of vascular cell proliferation; contributing to glucose homeostasis by enhancing pancreatic β-cell insulin secretion; triggering mitochondrial apoptosis during spermatocyte differentiation. Additionally, GPER1 stimulates uterine epithelial proliferation and oxytocin-responsive contractility, induces thymic atrophy via apoptosis, attenuates TNF-driven endothelial adhesion molecules, promotes hippocampal neuritogenesis, and protects against NMDA-induced neuronal death. In neuroendocrine regulation, it increases firing activity and calcium oscillations in LHRH neurons. During skeletal development, it suppresses growth plate osteoblast proliferation and inhibits adipocyte differentiation/lipid accumulation. In cancer contexts, GPER1 drives cancer-associated fibroblast (CAF) proliferation through EGFR/ERK signaling, potentially acts as a transcription factor with EGFR to activate growth genes (c-fos, cyclin D1), and enhances integrin α5β1-fibronectin matrix assembly in breast cancer cells.

Q99527

Predicted band size: 42 kDa; Observed band size: 55 kDa

Protein A

Non-conjugated

Unmodified

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Monoclonal

Rabbit

Human, Mouse, Rat

IHC: 1:1000-1:5000; WB: 1:2000-1:10000; ICC/IF: 1:200-1:1000; ELISA: 1:5000-1:20000; IP: 1:50-1:200

WB, IHC-P, ICC/IF, IP, ELISA

Liquid

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG