FOXP3 Antibody (YA759)

Cat. No.: HY-P80678: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
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FOXP3 Antibody (YA759) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to FOXP3.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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FOXP3 Antibody (YA759) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

FOXP3 Antibody (YA759) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to FOXP3.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 47 kDa;

Observed band size: 47 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q9BZS1

Gene ID

50943 [NCBI]

Immunogen

Synthetic peptide corresponding to Human FOXP3.The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Sensitivity	Transfected	Purity	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

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ALL IHC-P mIHC

Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using FOXP3 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80678, 1:1000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
Immunohistochemical analysis of paraffin-embedded human Liver Cancer tissue using FOXP3 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80678, 1:1000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Liver Cancer tissue using FOXP3 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80678, 1:1000 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with TSA520. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Liver Cancer tissue using FOXP3 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80678, 1:1000 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with TSA520. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

RRID

AB_3102662

Database

Entrez Gene: 50943 Human ; 20371 Mouse ; 317382 Rat

SwissProt: Q9BZS1 Human ; Q99JB6 Mouse ;

OMIM: 304790 Human

Research Field

Cell Biology

Synonyms

FOXP3; IPEX; JM2; Forkhead box protein P3; Scurfin

Documentation

Select Batch:

Data Sheet (232 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

FOXP3 Antibody (YA759) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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MedChemExpress Validation
Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using FOXP3 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80678, 1:1000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
MedChemExpress Validation
Immunohistochemical analysis of paraffin-embedded human Liver Cancer tissue using FOXP3 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80678, 1:1000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
MedChemExpress Validation
Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Liver Cancer tissue using FOXP3 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80678, 1:1000 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with TSA520. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
MedChemExpress Validation
Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Liver Cancer tissue using FOXP3 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80678, 1:1000 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with TSA520. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

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Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.

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FOXP3 Antibody (YA759)

FOXP3 Antibody (YA759) Featured Recommendations:

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FOXP3 Antibody (YA759) Related Classifications

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