ERG Antibody (YA2842)

ERG Antibody (YA2842) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2842), targeting ERG, with a predicted molecular weight of 54 kDa (observed band size: 54 kDa). ERG Antibody (YA2842) can be used for WB, IHC-P, ICC/IF, ChIP experiment in human, mouse, rat background.

ERG, a transcriptional regulator, is implicated in the modulation of gene expression by potentially facilitating transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure. Moreover, ERG has been identified as part of an IGF2BP1-dependent mRNP granule complex that contains untranslated mRNAs, suggesting its involvement in post-transcriptional processes. The interaction with SETDB1 underscores its role in the dynamic interplay of molecular components within cellular pathways, contributing to the intricate regulation of gene expression and chromatin organization.

P11308

Predicted band size: 54 kDa; Observed band size: 54 kDa

Affinity Chromatography

Non-conjugated

Unmodified

AB_3105213

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1/500-1/1000;IHC: 1/50-1/100;IF: 1/50-1/200;ChIP: 1/20

• 
  Western blot analysis was performed on extracts from Jurkat (lane 1, 20 μg), Raw264.7 (lane 2, 20 μg), Mouse brain (lane 3, 20 μg), and Rat brain (lane 4, 20 μg) using ERG Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

WB, IHC-P, ICC/IF, ChIP

Liquid

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human ERG