EphA2 Antibody (YA4830)

EphA2 Antibody (YA4830) is a mouse-derived and non-conjugated monoclonal antibody, targeting to EphA2. It can be applicated for WB, IHC-P, ICC/IF, ELISA assays, in the background of human.

The EphA2 protein, a receptor tyrosine kinase, engages in promiscuous binding to membrane-bound ephrin-A family ligands on adjacent cells, initiating contact-dependent bidirectional signaling. The downstream pathway originating from the receptor is known as forward signaling, while the pathway downstream of the ephrin ligand is termed reverse signaling. Activated by the ligand ephrin-A1/EFNA1, EphA2 plays a regulatory role in cell migration, integrin-mediated adhesion, proliferation, and differentiation. Additionally, EphA2 modulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibits the ERK1/ERK2 signaling pathway. It may also participate in UV radiation-induced apoptosis and exhibit a ligand-independent stimulatory effect on chemotactic cell migration. During development, EphA2 functions in various aspects of pattern formation and contributes to the development of several fetal tissues, including angiogenesis, early hindbrain development, and epithelial proliferation and branching morphogenesis during mammary gland development. Interaction with the ligand ephrin-A5/EFNA5 may regulate lens fiber cells' shape and interactions, playing a crucial role in lens transparency development and maintenance. Furthermore, in collaboration with ephrin-A2/EFNA2, EphA2 may contribute to bone remodeling by regulating osteoclastogenesis and osteoblastogenesis.

P29317

Predicted band size: 108 kDa;

affinity purified.

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human

WB: 1:500-1:2000; IHC: 1:200-1:1000; ELISA: 1:10000; ICC/IF: 1:50-200

WB, IHC-P, ICC/IF, ELISA

Liquid

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

Purified recombinant fragment of EphA2 expressed in E. Coli.