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  5. eIF4A3 Antibody (YA1822)

eIF4A3 Antibody (YA1822)

Cat. No.: HY-P82077
COA
SDS
User Guide for Antibodies Technical Support

eIF4A3 Antibody (YA1822) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA1822), targeting eIF4A3, with a predicted molecular weight of 47 kDa (observed band size: 47 kDa). eIF4A3 Antibody (YA1822) can be used for WB, IHC-P, ICC/IF, IP, FC experiment in human, mouse, rat background.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

eIF4A3 Antibody (YA1822) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA1822), targeting eIF4A3, with a predicted molecular weight of 47 kDa (observed band size: 47 kDa). eIF4A3 Antibody (YA1822) can be used for WB, IHC-P, ICC/IF, IP, FC experiment in human, mouse, rat background.
Background

eIF4A3 is an ATP-dependent RNA helicase involved in pre-mRNA splicing as a core component of the spliceosome. It serves as a core member of the splicing-dependent multiprotein exon junction complex (EJC), which is deposited at mRNA splice junctions. The EJC is a dynamic structure consisting of core proteins and transiently associated peripheral factors, marking exon-exon junctions and influencing downstream mRNA metabolism processes such as nuclear export, subcellular localization, translation efficiency, and nonsense-mediated mRNA decay (NMD). Its RNA-dependent ATPase and helicase activities are induced by CASC3 but inhibited by the MAGOH-RBM8A heterodimer, stabilizing the ATP-bound EJC core on spliced mRNA. eIF4A3 enhances translation of spliced mRNAs post-80S ribosome formation and binds mRNA sequence-independently 20-24 nucleotides upstream of exon-exon junctions, exhibiting higher RNA affinity in the ATP-bound EJC state. It also modulates splicing of apoptotic genes like BCL2L1/Bcl-X, specifically suppressing proapoptotic isoforms (e.g., Bcl-X(S)), a function distinct from canonical EJC assembly. Additionally, it plays a role in craniofacial development.
Gene ID
SwissProt ID
Synonyms
DDX48; EIF4A3; eIF4AIII; hNMP 265; NMP 265; NMP265; NUK34
Molecular Weight

Predicted band size: 47 kDa; Observed band size: 47 kDa
Purity

Affinity Chromatography
Conjugation

Non-conjugated
Modification

Unmodified
RRID
Research Field

Epigenetics and Nuclear Signaling
Product Categories

Primary Antibody; Recombinant Rabbit Monoclonal Antibody
Clonality

Recombinant, Monoclonal
Host

Rabbit
Reactivity

Human, Mouse, Rat
Dilution Ratio

WB: 1/500-1/1000;IHC: 1/50-1/100;IF: 1/50-1/200;IP: 1/40;FC: 1/50-1/100
Application

WB, IHC-P, ICC/IF, IP, FC
Appearance

Liquid
Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Isotype

IgG
Sensitivity

Endogenous
Immunogen

A synthesized peptide derived from human eIF4A3
Database

Entrez Gene: 9775 Human ; 192170 Mouse ; 688288 Rat

SwissProt: P38919 Human ; Q91VC3 Mouse ; Q3B8Q2 Rat

OMIM: 268305 Human

Documentation
SDS (251 KB)

COA (191 KB)

User Guide for Antibodies (1077 KB)

eIF4A3 Antibody (YA1822) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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eIF4A3 Antibody (YA1822)
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