2. Antibodies
  3. Primary Antibodies
  4. Monoclonal Antibodies
  5. eIF4A1 Antibody (YA774)

eIF4A1 Antibody (YA774)

Cat. No.: HY-P80650
COA User Guide for Antibodies Technical Support

eIF4A1 Antibody (YA774) is a non-conjugated and Mouse origined monoclonal antibody about 46 kDa, targeting to eIF4A1 (6D7). It can be used for WB,IHC-F,IHC-P,ICC/IF assays with tag free, in the background of Human, Mouse, Rat.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

eIF4A1 Antibody (YA774) is a non-conjugated and Mouse origined monoclonal antibody about 46 kDa, targeting to eIF4A1 (6D7). It can be used for WB,IHC-F,IHC-P,ICC/IF assays with tag free, in the background of Human, Mouse, Rat.
Background

eIF4A1 (6D7): Enables double-stranded RNA binding activity. Predicted to be involved in cytoplasmic translational initiation. Located in cytoplasm. [provided by Alliance of Genome Resources, Apr 2022]
Tag

Free
Gene ID
SwissProt ID
Synonyms
Eukaryotic initiation factor 4A-I; eIF-4A-I; eIF4A-I; ATP-dependent RNA helicase eIF4A-1
Molecular Weight

Predicted band size: 46 kDa
Purity

affinity purified
Conjugation

Non-conjugated
Modification

Unmodified
RRID
Research Field

Epigenetics and Nuclear Signaling
Product Categories

Primary Antibody; Mouse Monoclonal Antibody
Clonality

Monoclonal
Host

Mouse
Reactivity

Human, Mouse, Rat
Dilution Ratio

WB: 1:500-1:1,000 ;IHC: 1:50-1:100 ;IF: 1:50-1:200

  • Western blot analysis of extracts from 293T (lane 1) and Hela (lane 2) using eIF4A1 Antibody (YA774) antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
Application

WB, IHC-F, IHC-P, ICC/IF
Appearance

Liquid
Formulation

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Isotype

IgG1
Sensitivity

Endogenous
Immunogen

Synthetic peptide corresponding to eIF4A1.The exact sequence is proprietary to MCE.
Database

Entrez Gene: 1973 Human ; 13681 Mouse ; 287436 Rat

SwissProt: P60842 Human ; P60843 Mouse ;

OMIM: 602641 Human

Documentation

COA (191 KB)

User Guide for Antibodies (1077 KB)

eIF4A1 Antibody (YA774) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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eIF4A1 Antibody (YA774)
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