DDX5 Antibody (YA2666)

DDX5 Antibody (YA2666) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to DDX5.

DDX5 is an RNA helicase involved in the alternative regulation of pre-mRNA splicing, where its helicase activity promotes tau exon 10 inclusion in an RBM4-dependent manner. It binds to the stem-loop region downstream of exon 10 in tau pre-mRNA, with ATP hydrolysis rates highly stimulated by single-stranded RNA. Beyond splicing, DDX5 participates in transcription regulation (independent of helicase activity), acting as a coactivator for androgen receptor AR (but not ESR1) and synergizing with DDX17 and SRA1 RNA to activate MYOD1 transcriptional activity during skeletal muscle differentiation. It also serves as a coactivator for p53/TP53 in DNA damage response and apoptosis, and for RUNX2 in osteoblast differentiation. Additionally, DDX5 exhibits promoter-specific transcriptional repression likely through HDAC1 interaction, and as part of a PER complex, inhibits 3' transcriptional termination of circadian genes like PER1 and NR1D1 to modulate circadian rhythms.

P17844

Predicted band size: 69 kDa; Observed band size: 69 kDa

Affinity Purified

Non-conjugated

Unmodified

AB_3105013

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-1:1000; IHC: 1:50-1:100; IF: 1:50-1:200; IP: 1:20

• 
  Immunohistochemical analysis of paraffin-embedded mouse brain tissue using DDX5 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded mouse brain tissue using DDX5 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-F, IHC-P, ICC/IF, IP

Liquid

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthetic peptide of human DDX5