DDX1 Antibody (YA4396)

DDX1 Antibody (YA4396) is a mouse-derived and non-conjugated IgG1 antibody, targeting to DDX1. It can be applicated for WB, IHC-P, ICC/IF, FC, ELISA assays, in the background of human, mouse.

DDX1 is an ATP-dependent RNA helicase capable of unwinding both RNA-RNA and RNA-DNA duplexes. It exhibits 5' single-stranded RNA overhang nuclease activity and ATPase activity on various RNA (but not DNA) polynucleotides. It may play a role in RNA clearance at DNA double-strand breaks (DSBs), facilitating template-guided repair of transcriptionally active genomic regions. Acting as a coactivator with RELA, it enhances NF-kappa-B-mediated transcriptional activation. It positively regulates cyclin CCND2 expression by binding to its promoter region and associates with chromatin at the NF-kappa-B promoter via RELA interaction. It binds poly(A) RNA and may participate in 3'-end cleavage and polyadenylation of pre-mRNAs. As part of the tRNA-splicing ligase complex, it promotes RTCB guanylylation (a key step in tRNA ligation) alongside archease (ZBTB8OS). It is a component of a multi-helicase-TICAM1 complex that senses viral dsRNA, triggering antiviral responses (including pro-inflammatory cytokine induction) via TICAM1. It specifically binds (via its helicase ATP-binding domain) to short and long poly(I:C) dsRNA (by similarity). In microbial infections, it is required for HIV-1 Rev function and the replication of HIV-1 (binding HIV-1 mRNA RRE sequences) and coronavirus IBV.

Q92499

Predicted band size: 82 kDa; Observed band size: 82 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Mouse

Human, Mouse

WB: 1/500-1/2000; IHC: 1/200-1/1000; IF: 1/200-1/1000; FC: 1/200-1/400; ELISA: 1/10000

WB, IHC-P, ICC/IF, FC, ELISA

Liquid

Supplied in PBS with 0.05% sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Purified recombinant fragment of human DDX1 (AA: 642-740) expressed in E. Coli.