DAB2 Antibody (YA2852)

DAB2 Antibody (YA2852) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2852), targeting DAB2, with a predicted molecular weight of 82 kDa (observed band size: 96 kDa). DAB2 Antibody (YA2852) can be used for WB, IHC-P, IP experiment in human background.

DAB2 is an adapter protein functioning as a clathrin-associated sorting protein (CLASP) essential for clathrin-mediated endocytosis of specific cargo proteins. It binds and assembles clathrin while simultaneously interacting with phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) and cargo proteins containing non-phosphorylated NPXY internalization motifs (e.g., LDL receptor), recruiting them to clathrin-coated pits. DAB2 operates independently of the AP-2 complex in clathrin-mediated endocytosis and participates in integrin β-1 endocytosis (a redundant function with AP-2 requiring DAB2’s binding to EH domain-containing proteins like EPS15, EPS15L1, and ITSN1). It also mediates endocytosis of cystic fibrosis transmembrane conductance regulator (CFTR) and megalin/LRP2 lipoprotein receptor during embryogenesis, as well as TGF-β receptor recycling. DAB2 facilitates CFTR trafficking to late endosomes and engages in multiple receptor signaling pathways, including TGF-β receptor signaling (promoting SMAD2 phosphorylation) and TGF-β-stimulated JNK activation. It may inhibit canonical Wnt/β-catenin signaling by stabilizing the β-catenin destruction complex (via competitive axin binding to block PP1-mediated dephosphorylation) or sequestering LRP6 via clathrin endocytosis, while potentially activating non-canonical Wnt pathways. In growth factor/Ras signaling, DAB2 suppresses ERK activation by disrupting GRB2-SOS1 interaction and inhibits SRC by preventing its phosphorylation at Tyr-419. It may modulate androgen receptor (AR) signaling by competing with AR for SRC binding and contributes to CSF-1 signal transduction, cellular differentiation, visceral endoderm (VE) formation during embryogenesis (responding to epiblast-derived Nodal signals), and epithelial-mesenchymal transition (EMT). Additionally, DAB2 might regulate myeloid differentiation, induce macrophage adhesion/spreading, and act as a tumor suppressor.

P98082

Predicted band size: 82 kDa; Observed band size: 96 kDa

Affinity Purified

Non-conjugated

Unmodified

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1/500-1/1000;IHC: 1/50-1/100;IP: 1/20

WB, IHC-P, IP

Liquid

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthetic peptide of human DAB2