Cytokeratin 19 Antibody

Cytokeratin 19 Antibody is an unconjugated, approximately 44 kDa, rabbit-derived, anti-Cytokeratin 19 polyclonal antibody. Cytokeratin 19 Antibody can be used for: WB, ELISA, IHC-P, IHC-F, Flow-Cyt, IF expriments in human and predicted: mouse, rat, chicken, dog, pig, cow, horse, rabbit background without labeling.

Cytokeratin is a protein involved in the organization of myofibers. Together with KRT8, it helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

P08727

Predicted band size: 42 kDa; Observed band size: 42 kDa

affinity purified

Non-conjugated

Unmodified

AB_3103090

Primary Antibody; Rabbit Polyclonal Antibody

Polyclonal

Rabbit

Human

Mouse, Rat, Chicken, Pig, Cow, Dog, Horse

WB: 1:500-2000;ELISA: 1:5000-10000;IHC-P: 1:100-500;IHC-F: 1:100-500;Flow-Cyt: 1μg /Test;IF: 1:100-500

• 
  Immunofluorescence analysis of MCF7 cells labeling Cytokeratin 19 with Cytokeratin 19 (HY-P81183) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA/10% normal goat serum for 1 hour at room temperature. Cells were then incubated with Cytokeratin 19 (HY-P81183) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Alexa Fluor® 647-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P80952) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
• 
  Immunofluorescence analysis of HEC1A cells labeling Cytokeratin 19 with Cytokeratin 19 (HY-P81183) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA/10% normal goat serum for 1 hour at room temperature. Cells were then incubated with Cytokeratin 19 (HY-P81183) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Alexa Fluor® 647-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P80952) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

WB, ELISA, IHC-P, IHC-F, FC, ICC/IF

Liquid

Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

KLH conjugated synthetic peptide derived from human Cytokeratin 19.: 101-200/403