Cytokeratin 17 Antibody (YA3438)

Cat. No.: HY-P83702: Data Sheet COA
SDS
User Guide for Antibodies
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Technical Support

Cytokeratin 17 Antibody (YA3438) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cytokeratin 17.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Cytokeratin 17 Antibody (YA3438) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

Cytokeratin 17 Antibody (YA3438) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cytokeratin 17.

Host

Rabbit

Clonality

Monoclonal, Recombinant

Molecular Weight

Predicted band size: 48 kDa;

Observed band size: 48 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q04695

Gene ID

3872 [NCBI]

Immunogen

Synthetic peptide within Human Cytokeratin 17 aa 1-50 / 432.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:200
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:1500

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

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ALL WB ICC

Western blot analysis of extracts from A431 (lane 2(20μg) , A431(lane 3(40μg),using Cytokeratin 17 Antibody (HY-P83702). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
Immunocytochemistry analysis of HeLa cells labeling Cytokeratin 17 with Cytokeratin 17 Antibody (HY-P83702) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Cytokeratin 17 Antibody (HY-P83702) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of SK-BR-3 cells labeling Cytokeratin 17 with Cytokeratin 17 Antibody (HY-P83702) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Cytokeratin 17 Antibody (HY-P83702) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

RRID

AB_3676585

Database

Entrez Gene: 3872 Human ; 16667 Mouse ; 287702 Rat

SwissProt: Q04695 Human ; Q9QWL7 Mouse ; Q6IFU8 Rat

OMIM: 167210 Human

Synonyms

39.1 antibody; CK 17 antibody; CK17 antibody; CK-17 antibody; Cytokeratin-17 antibody; K17 antibody; K1C17_HUMAN antibody; Keratin 17 antibody; keratin 17 epitope S1 antibody; keratin 17 epitope S2 antibody; keratin 17 epitope S4 antibody; Keratin 17, type I antibody; Keratin antibody; Keratin type I cytoskeletal 17 antibody; keratin, type i cytoskeletal 17 [version 1] antibody; Keratin-17 antibody; KRT17 antibody; PC antibody; PC2 antibody; PCHC1 antibody; type I cytoskeletal 17 antibody;

Documentation

Select Batch:

Data Sheet (235 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Cytokeratin 17 Antibody (YA3438) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Cytokeratin 17 Antibody (YA3438)

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