Cathepsin K Antibody

Cathepsin K Antibody is an unconjugated, approximately 36 kDa, rabbit-derived, anti-Cathepsin K polyclonal antibody. Cathepsin K Antibody can be used for: WB, ELISA, IHC-P, IHC-F, IF expriments in rat, and predicted: human, mouse, dog, pig, cow, horse, guinea pig background without labeling.

Cathepsin K Protein, a thiol protease, assumes a crucial role in osteoclastic bone resorption, contributing to the intricate process of bone remodeling. This enzyme exhibits potent endoprotease activity, particularly against fibrinogen, under acidic conditions, suggesting its involvement in extracellular matrix degradation. Furthermore, Cathepsin K plays a role in the regulated release of thyroid hormone thyroxine (T4) through limited proteolysis of TG/thyroglobulin within the thyroid follicle lumen, highlighting its multifaceted functions in physiological processes.

P43235

Predicted band size: 36 kDa; Observed band size: 46 kDa

affinity purified

Lysosome.

Non-conjugated

Unmodified

AB_3103080

Primary Antibody; Rabbit Polyclonal Antibody

Polyclonal

Rabbit

Rat

Human, Mouse, Dog, Pig, Cow, Horse, GuineaPig

WB: 1:500-2000;ELISA: 1:5000-10000;IHC-P: 1:100-500;IHC-F: 1:100-500;IF: 1:100-500

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  Western blot analysis of extracts from Heart (lane 1) and Muscle (lane 2) using Cathepsin K antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

WB, ELISA, IHC-P, IHC-F, ICC/IF

Liquid

Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

KLH conjugated synthetic peptide derived from human Cathepsin K: 112-210/329