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  4. Casein Kinase 1 alpha Antibody (YA564)

Casein Kinase 1 alpha Antibody (YA564)

Cat. No.: HY-P80585
COA User Guide for Antibodies Technical Support

Casein Kinase 1 alpha Antibody (YA564) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Casein Kinase 1 alpha.

For research use only. We do not sell to patients.

Size Price Stock Quantity
10 μL In-stock
50 μL In-stock
100 μL In-stock
250 μL   Get quote  

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Top Publications Citing Use of Products

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

Casein Kinase 1 alpha Antibody (YA564) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Casein Kinase 1 alpha.

Background

Casein Kinase 1 alpha: Enables protein serine/threonine kinase activity. Involved in several processes, including negative regulation of canonical Wnt signaling pathway; peptidyl-serine phosphorylation; and positive regulation of proteasomal ubiquitin-dependent protein catabolic process. Located in centrosome; cytosol; and nuclear speck. Part of beta-catenin destruction complex. Colocalizes with keratin filament and mRNA cleavage and polyadenylation specificity factor complex. Biomarker of Alzheimer's disease and inclusion body myositis. [provided by Alliance of Genome Resources, Apr 2022]

Tag

Free

Gene ID
SwissProt ID
Synonyms
CSNK1A1; Casein kinase I isoform alpha; CKI-alpha; CK1
Molecular Weight

Predicted band size: 39 kDa; Observed band size: 34 kDa

Purity

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

RRID
Research Field

Signal Transduction

Product Categories

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Clonality

Recombinant, Monoclonal

Host

Rabbit

Reactivity

Human, Rat

Dilution Ratio

WB: 1:500-1:1000; IP: 1:20

  • Western blot analysis of extracts from K562(lane 2(20μg) and K562(lane 3(40μg) using Casein Kinase 1 alpha (HY-P80585) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Application

WB, IP

Appearance

Liquid

Formulation

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Isotype

IgG

Sensitivity

Endogenous

Immunogen

Synthetic peptide corresponding to Human Casein Kinase 1 alpha.The exact sequence is proprietary to MCE.

Database
Documentation

Casein Kinase 1 alpha Antibody (YA564) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Casein Kinase 1 alpha Antibody (YA564)
Cat. No.:
HY-P80585
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