BRCA1 Antibody (YA3986)

BRCA1 Antibody (YA3986) is a mouse-derived and non-conjugated IgG1 antibody, targeting to BRCA1. It can be applicated for IHC-P, ELISA assays, in the background of human.

BRCA1 is an E3 ubiquitin-protein ligase that specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage. It is unclear whether it also mediates the formation of other types of polyubiquitin chains. The BRCA1-BARD1 heterodimer coordinates diverse cellular pathways such as DNA damage repair, ubiquitination, and transcriptional regulation to maintain genomic stability. Additionally, it regulates centrosomal microtubule nucleation and is required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and G2 phase. BRCA1 is also involved in targeting FANCD2 to sites of DNA damage, inhibits lipid synthesis by binding to inactive phosphorylated ACACA, and contributes to homologous recombination repair (HRR) through its interaction with PALB2. As part of the BRCA1-RBBP8 complex, it regulates CHEK1 activation and controls the G2/M checkpoint following DNA damage via BRCA1-mediated ubiquitination of RBBP8. BRCA1 also functions as a transcriptional activator.

P38398

Predicted band size: 208 kDa; Observed band size: 208 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Mouse

Human

IHC: 1/200-1/1000; ELISA: 1/10000

IHC-P, ELISA

Liquid

Supplied in PBS with 0.05% sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Purified recombinant fragment of human BRCA1 (AA: 229-335) expressed in E. Coli.