ATM Antibody (YA4232)

ATM Antibody (YA4232) is a mouse-derived and non-conjugated IgG2b antibody, targeting to ATM. It can be applicated for IHC-P, ICC/IF, FC, ELISA assays, in the background of human, mouse, monkey, rat.

ATM is a serine/threonine protein kinase that activates checkpoint signaling in response to double-strand breaks (DSBs), apoptosis, and genotoxic stresses such as ionizing radiation, functioning as a DNA damage sensor. It recognizes the substrate consensus sequence [ST]-Q and phosphorylates histone variant H2AX at Ser-139 at DSBs, thereby regulating the DNA damage response mechanism (By similarity). Additionally, ATM plays a role in pre-B cell allelic exclusion by repositioning immunoglobulin heavy chain alleles to enforce clonality. It is involved in signal transduction, cell cycle control, and may act as a tumor suppressor. ATM phosphorylates various substrates, including DYRK2, CHEK2, p53/TP53, BRCA1, etc., modulating their functions. Under genotoxic stress, ATM prevents ubiquitin-mediated degradation of DYRK2 and enhances the DNA damage response by phosphorylating ATF2 and ERCC6. Furthermore, ATM participates in pexophagy by phosphorylating PEX5, promoting its ubiquitination and degradation.

Q13315

Predicted band size: 351 kDa; Observed band size: 370 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Mouse

Human, Mouse, Monkey, Rat

IHC: 1/200-1/1000; IF: 1/200-1/1000; FC: 1/200-1/400; ELISA: 1/10000

IHC-P, ICC/IF, FC, ELISA

Liquid

Supplied in PBS with 0.05% sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG2b

Purified recombinant fragment of human ATM (AA: 2577-3056) expressed in E. Coli.