ATF4 Antibody (YA605)

ATF4 Antibody (YA605) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ATF4.

ATF4 is a transcription factor that binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3') and functions as a regulator of metabolic and redox processes under normal cellular conditions, as well as a master transcription factor during the integrated stress response (ISR). It binds to asymmetric CREs as a heterodimer and to palindromic CREs as a homodimer (By similarity). As a core effector of ISR, ATF4 is essential for adaptation to various stresses, including endoplasmic reticulum (ER) stress, amino acid starvation, mitochondrial stress, and oxidative stress. During ISR, ATF4 translation is induced via an alternative ribosome translation re-initiation mechanism in response to EIF2S1/eIF-2-alpha phosphorylation, and stress-induced ATF4 acts as a master regulator of stress-responsive genes to promote cell recovery. It also activates the transcription of genes linked to amino acid sufficiency and oxidative stress resistance (By similarity). In ER stress, ATF4 may cooperate with other factors to activate NLRP1 transcription and induces asparagine synthetase (ASNS) expression in response to amino acid deprivation or ER stress. However, when associated with DDIT3/CHOP, ATF4-mediated ASNS transcription is inhibited under amino acid deprivation. ATF4, together with DDIT3/CHOP, promotes programmed cell death by regulating genes involved in cellular amino acid metabolism, mRNA translation, and the terminal unfolded protein response (terminal UPR) (By similarity). Additionally, ATF4 activates COX7A2L/SCAF1 expression downstream of the EIF2AK3/PERK-mediated UPR, enhancing respiratory chain supercomplex formation and mitochondrial oxidative phosphorylation. Under non-stress conditions, ATF4 is involved in embryonic lens formation, fetal liver hematopoiesis, bone development, and synaptic plasticity (By similarity). In osteoblasts, ATF4 phosphorylation by RPS6KA3/RSK2 enhances its transactivation activity and promotes osteoblast-specific gene expression. ATF4 also cooperates with FOXO1 to regulate glucose homeostasis by suppressing β-cell production and insulin secretion (By similarity). Furthermore, ATF4 activates SIRT4 transcription and regulates circadian expression of PER2 and SLC6A4 by periodically binding to CREs in their promoters (By similarity). While primarily functioning as a transcriptional activator in stress adaptation, ATF4 can also act as a repressor, inhibiting long-term memory formation. In neurons, DISC1 interaction disrupts ATF4 dimerization and DNA-binding, suppressing its transcriptional activity (Microbial infection) ATF4 binds to a Tax-responsive enhancer element in the HTLV-I long terminal repeat.

Free

P18848

Predicted band size: 39 kDa；Observed band size: 55 kDa

Protein A affinity purified.

Nucleus, Nucleus speckle, Cell membrane, Cytoplasm, Centrosome.

Non-conjugated

Unmodified

AB_3102559

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-1:2,000; ICC/IF: 1:50-1:200; IHC-P: 1:50-1:200; FC: 1:50-1:100; IP: Use at an assay dependent concentration.

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  Immunocytochemistry analysis of Neuro-2a cells labeling ATF4 with ATF4 Antibody (HY-P80486) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ATF4 Antibody (HY-P80486) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of Neuro-2a cells labeling ATF4 with ATF4 Antibody (HY-P80486) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ATF4 Antibody (HY-P80486)at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunohistochemical analysis of paraffin-embedded rat colon tissue using ATF4 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80486, 1:400) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded rat colon tissue using ATF4 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80486, 1:400) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, ICC/IF, IHC-P, IP, FC

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human ATF4.AA range:1-220.