Arrestin-β-2 Antibody (YA5111)

Arrestin-β-2 Antibody (YA5111) is a mouse-derived and non-conjugated monoclonal antibody, targeting to Arrestin-β-2. It can be applicated for WB, ICC/IF assays, in the background of human, mouse, rat, pig.

Arrestin-β-2 is a multifunctional protein that primarily regulates G-protein coupled receptor (GPCR) signaling by mediating receptor desensitization and resensitization. It binds to GPRK-phosphorylated receptors, sterically hindering their coupling to G-proteins (homologous desensitization). Additionally, as an endocytic adapter (CLASPs), it recruits GPCRs to the AP-2 complex in clathrin-coated pits (CCPs) to promote receptor internalization. The extent of Arrestin-β-2 involvement varies significantly depending on receptor type, agonist, and cell context. It also mediates internalization of non-GPCR receptors such as TGFBR2, LDLR, and ACKR3. Acting as a signaling scaffold, Arrestin-β-2 activates MAPK pathways (e.g., ERK1/2 and JNK3) and AKT1, though the activated ERK1/2 and JNK3 are largely confined to cytoplasmic endocytic vesicles (beta-arrestin signalosomes). It further modulates inflammatory responses, insulin resistance, and inhibitory signaling in natural killer cells. by similar: Arrestin-β-2 and Arrestin-β-1 (ARRB1) exhibit codependent regulation in certain GPCR pathways (e.g., ADRB2, F2RL1, PTH1R), while in others, one arrestin form may dominate while the other inhibits (reciprocal regulation).

P32121

Predicted band size: 46 kDa;

affinity purified.

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Mouse, Rat, Pig

WB: 1:1000-1:2000; ICC/IF: 1:100-1:500

WB, ICC/IF

Liquid

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

Purified recombinant human Arrestin-β-2 (C-terminus) protein fragments expressed in E.coli.