APC Antibody (YA617)

APC Antibody (YA617) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to APC.

APC Protein serves as a tumor suppressor with multifaceted functions. It facilitates the rapid degradation of CTNNB1 and acts as a negative regulator in the Wnt signaling pathway. The activity of APC is intricately tied to its phosphorylation state. Additionally, it activates the GEF activity of SPATA13 and ARHGEF4, participating in hepatocyte growth factor (HGF)-induced cell migration and playing a role in MMP9 up-regulation through the JNK signaling pathway in colorectal tumor cells. Beyond its involvement in signaling pathways, APC associates with microtubules and actin filaments, components of the cytoskeleton, contributing to the organization of F-actin into ordered bundles. It functions downstream of Rho GTPases and DIAPH1, selectively stabilizing microtubules and mediating ERBB2-dependent stabilization of microtubules at the cell cortex. APC is required for the localization of MACF1 to the cell membrane, a critical factor in its function related to microtubule stabilization. It forms homooligomers and is found in complexes with various proteins, including ARHGEF4, CTNNB1, AXIN1, GSK3B, JPT1, APC2, DLG1, DLG3, alpha- and beta-catenins, MAPRE1, MAPRE2, MAPRE3, DIAPH1, DIAPH2, SCRIB, SPATA13, ASAP1, AMER1, AMER2, and KHDRBS1. APC's interaction with actin involves binding to both F-actin and actin filament bundles. This comprehensive network of interactions highlights the diverse roles of APC in cellular processes and its significance as a tumor suppressor.

Free

P25054

Predicted band size: 312 kDa; Observed band size: 160 kDa

Protein A affinity purified.

Cytoplasm, Cell junction, Cell membrane.

Non-conjugated

Unmodified

AB_3102802

Cardiovascular

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse

WB: 1:1000-1:5000; ICC: 1:50-1:200

WB, ICC/IF, IP

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human APC.AA range:51-100.