2. Antibodies
  3. Secondary Antibodies
  4. Enzyme and Protein Conjugated Secondary Antibodies Spatial Conformation Specific Secondary Antibodies
  5. HRP-conjugated Anti-Rabbit IgG for IP

HRP-conjugated Anti-Rabbit IgG for IP

Cat. No.: HY-P87496
COA
SDS
User Guide for Antibodies Technical Support

Anti-Rabbit IgG for IP (HRP) is a conformation-specific secondary antibody that binds exclusively to native, non-denatured IgG and does not recognize denatured IgG. Conformation-specific secondary antibodies target spatial epitopes. When antibodies are denatured by high temperatures, they lose their native conformation and are no longer recognized by such antibodies—neither the heavy chain nor the light chain is detected. This effectively avoids interference from the heavy chain (55 kDa) and light chain (25 kDa) in immunoprecipitation (IP) experiments.

For research use only. We do not sell to patients.

Size Price Stock Quantity
50 μL In-stock
100 μL In-stock
250 μL   Get quote  
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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Documentation

Description

Anti-Rabbit IgG for IP (HRP) is a conformation-specific secondary antibody that binds exclusively to native, non-denatured IgG and does not recognize denatured IgG. Conformation-specific secondary antibodies target spatial epitopes. When antibodies are denatured by high temperatures, they lose their native conformation and are no longer recognized by such antibodies—neither the heavy chain nor the light chain is detected. This effectively avoids interference from the heavy chain (55 kDa) and light chain (25 kDa) in immunoprecipitation (IP) experiments.
Host

Mouse
Clonality

Monoclonal
Species Reactivity
Rabbit
Immunogen

Rabbit IgG
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:2500-1:10000
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
1:1000-1:3000
Appearance

Liquid
Formulation

Supplied in phosphate Buffered Saline containing 1% BSA, 0.01% thimerosal, 50% glycerol, pH 7.4.
Storage & Stability

Stored at -20°C for 1 year.Protect from light. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Documentation
SDS (251 KB)

COA (204 KB)

User Guide for Antibodies (1077 KB)

HRP-conjugated Anti-Rabbit IgG for IP Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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HRP-conjugated Anti-Rabbit IgG for IP
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HY-P87496
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