Anterior Gradient 2 Antibody (YA1779)

Anterior Gradient 2 Antibody (YA1779) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA1779), targeting Anterior Gradient 2, with a predicted molecular weight of 20 kDa (observed band size: 20 kDa). Anterior Gradient 2 Antibody (YA1779) can be used for WB, IHC-F, IHC-P, ICC/IF, IP experiment in human, rat background.

Anterior Gradient 2 (AGR2) is a proto-oncogene involved in cell migration, differentiation, and growth, and it promotes cell adhesion. It is also required for the post-transcriptional synthesis and secretion of MUC2 and may play a role in mucus production by intestinal cells (By similarity).

O95994

Predicted band size: 20 kDa; Observed band size: 20 kDa

Affinity Purified

Non-conjugated

Unmodified

AB_3104155

Cancer

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Rat

WB: 1/500-1/1000;IHC: 1/50-1/100;IF: 1/50-1/200;IP: 1/20

• 
  Western blot analysis of extracts from K562 (lane 2(20μg), C6 (lane 3(20μg) and MCF-7 (lane 4 (20μg) using Anterior Gradient 2 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of Hela cells labeling Anterior Gradient 2 with Anterior Gradient 2 antibody (HY-P82034) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Anterior Gradient 2 antibody (HY-P82034) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of NIH/3T3 cellslabeling Anterior Gradient 2 with Anterior Gradient 2 antibody (HY-P82034) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Anterior Gradient 2 antibody (HY-P82034) at 1/200 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

WB, IHC-F, IHC-P, ICC/IF, IP

Liquid

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthetic peptide of human Anterior Gradient 2