Alkaline phosphatase Antibody (YA6345)

Alkaline phosphatase Antibody (YA6345) is a rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Alkaline phosphatase. It can be applicated for WB assays, in the background of human, mouse, rat.

Alkaline Phosphatase (ALP) is an enzyme that metabolizes various phosphate compounds and plays a crucial role in skeletal mineralization and adaptive thermogenesis. It exhibits broad substrate specificity, hydrolyzing a wide range of compounds, though its natural substrates primarily include diphosphate (inorganic pyrophosphate, PPi), pyridoxal 5’-phosphate (PLP), and N-phosphocreatine. By hydrolyzing extracellular diphosphate—a potent mineralization inhibitor—into phosphate, ALP promotes hydroxyapatite crystal formation and increases inorganic phosphate concentration, thereby playing a vital role in skeletal and dental mineralization (By similarity). It functions non-redundantly with PHOSPHO1 in skeletal mineralization: while PHOSPHO1 initiates hydroxyapatite crystallization in matrix vesicles (MVs), ALPL/TNAP facilitates its propagation. Additionally, ALP catalyzes the dephosphorylation of PLP to pyridoxal (PL), the transportable form of vitamin B6, ensuring sufficient PLP availability in the brain as a cofactor for neurotransmitter-synthesizing enzymes. The enzyme also mediates stepwise ATP degradation to adenosine, regulating purinergic receptor ligand availability (By similarity), and dephosphorylates microbial products (e.g., lipopolysaccharides, LPS) and phosphorylated small molecules (e.g., poly-inosine:cytosine, poly I:C). In adaptive thermogenesis, ALP acts as a key regulator in the futile creatine cycle by hydrolyzing N-phosphocreatine to initiate a cycle of creatine dephosphorylation and phosphorylation, dissipating energy as heat without performing mechanical or chemical work (By similarity).

P05186

Predicted band size: 56 kDa

affinity purified.

Cell membrane; Lipid-anchor, GPI-anchor.

Non-conjugated

Unmodified

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-2000

WB

Liquid

Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

KLH conjugated synthetic peptide derived from human Alkaline phosphatase, tissue-nonspecific isozyme aa 18-50