ABCG5 Antibody (YA4488)

ABCG5 Antibody (YA4488) is a mouse-derived and non-conjugated IgG1 antibody, targeting to ABCG5. It can be applicated for WB, IHC-P, FC, ELISA assays, in the background of human.

ABCG5 and ABCG8 form an obligate heterodimer that mediates Mg²⁺- and ATP-dependent sterol transport across membranes. It plays a vital role in the selective transport of dietary plant sterols and cholesterol in enterocytes and biliary sterol excretion (by similar). The heterodimer is also essential for sterol homeostasis and exhibits ATPase activity (by similar).

Q9H222

Predicted band size: 72.5 kDa; Observed band size: 32 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Mouse

Human

WB: 1/500-1/2000; IHC: 1/200-1/1000; FC: 1/200-1/400; ELISA: 1/10000

• 
  Western blot analysis of extracts from Jurkat (lane 2(20μg), Jurkat (lane 3(40μg), using ABCG5 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

WB, IHC-P, FC, ELISA

Liquid

Supplied in PBS with 0.05% sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Purified recombinant fragment of human ABCG5 (AA: 306-367) expressed in E. Coli.