ABCG2 Antibody (YA4578)

ABCG2 Antibody (YA4578) is a mouse-derived and non-conjugated IgG1 antibody, targeting to ABCG2. It can be applicated for WB, IHC-P, ICC/IF, FC, ELISA assays, in the background of human, mouse, rat, rabbit, monkey.

ABCG2 is an ATP-dependent transporter with broad substrate specificity belonging to the ATP-binding cassette (ABC) family, which actively extrudes a wide variety of physiological compounds, dietary toxins and xenobiotics from cells; it is involved in porphyrin homeostasis, mediating the export of protoporphyrin IX (PPIX) from mitochondria to cytosol and from cytosol to extracellular space, and also functions in the cellular export of heme; it mediates the efflux of sphingosine-1-P from cells; acts as a urate exporter involved in both renal and extrarenal urate excretion; in the kidney, it also functions as a physiological exporter of the uremic toxin indoxyl sulfate (By similarity); is involved in the excretion of steroids such as estrone 3-sulfate (E1S), 3beta-sulfooxy-androst-5-en-17-one (DHEAS), and other sulfate conjugates; mediates the secretion of vitamins like riboflavin and biotin into milk (By similarity); extrudes pheophorbide a, a phototoxic porphyrin catabolite of chlorophyll, reducing its bioavailability (By similarity); plays an important role in the exclusion of xenobiotics from the brain (Probable); confers cells resistance to multiple drugs and other xenobiotics including mitoxantrone, pheophorbide, camptothecin, methotrexate, azidothymidine, and the anthracyclines daunorubicin and doxorubicin by controlling their efflux; in the placenta, limits the penetration of drugs from the maternal plasma into the fetus (By similarity); may play a role in early stem cell self-renewal by blocking differentiation (By similarity); in inflammatory macrophages, exports itaconate from the cytosol to the extracellular compartment and limits the activation of TFEB-dependent lysosome biogenesis involved in antibacterial innate immune response.

Q9UNQ0

Predicted band size: 72 kDa; Observed band size: 50-70 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Mouse

Human, Mouse, Rat, Rabbit, Monkey

WB: 1/500-1/2000; IHC: 1/100-1/500; IF: 1/50-1/500; FC: 1/200-1/400; ELISA: 1/10000

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  Western blot analysis of extracts from Hela (lane 2(20μg), Hela (lane 3(40μg), using ABCG2 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

WB, IHC-P, ICC/IF, FC, ELISA

Liquid

Supplied in PBS with 0.05% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Purified recombinant fragment of human ABCG2 expressed in E. Coli.