15 Lipoxygenase 1/ALOX15 Atibody (YA1091)

15 Lipoxygenase 1/ALOX15 Antibody (YA1091) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to 15 Lipoxygenase 1/ALOX15 .

15 Lipoxygenase 1 is a non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids, generating a spectrum of bioactive lipid mediators; it inserts peroxyl groups at C12 or C15 of arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate), producing both 12-hydroperoxyeicosatetraenoate (12-HPETE) and 15-hydroperoxyeicosatetraenoate (15-HPETE), and may then act on 12-HPETE to produce hepoxilins, which may show pro-inflammatory properties (By similarity); it can also peroxidize linoleate ((9Z,12Z)-octadecadienoate) to 13-hydroperoxyoctadecadienoate (13-HPODE), and may participate in the sequential oxidations of DHA ((4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate) to generate specialized pro-resolving mediators (SPMs) like resolvin D5 ((7S,17S)-diHPDHA) and (7S,14S)-diHPDHA, which actively down-regulate the immune response and have anti-aggregation properties with platelets; it can convert epoxy fatty acids to hydroperoxy-epoxides derivatives followed by an intramolecular nucleophilic substitution leading to the formation of monocyclic endoperoxides; it plays an important role during the maintenance of self-tolerance by peroxidizing membrane-bound phosphatidylethanolamine, which can then signal the sorting process for clearance of apoptotic cells during inflammation and prevent an autoimmune response; in addition to its role in the immune and inflammatory responses, this enzyme may play a role in epithelial wound healing in the cornea through production of lipoxin A4 (LXA(4)) and docosahexaenoic acid-derived neuroprotectin D1 (NPD1; 10R,17S-HDHA), both lipid autacoids exhibit anti-inflammatory and neuroprotective properties; furthermore, it may regulate actin polymerization, which is crucial for several biological processes such as the phagocytosis of apoptotic cells, and is also implicated in the generation of endogenous ligands for peroxisome proliferator activated receptor (PPAR-gamma), hence modulating macrophage development and function, and may also exert a negative effect on skeletal development by regulating bone mass through this pathway; it also participates in ER stress and downstream inflammation in adipocytes, pancreatic islets, and liver (By similarity); finally, it is also involved in the cellular response to IL13/interleukin-13.

P16050

Predicted band size: 75 kDa; Observed band size: 75 kDa

Affinity Purified

Non-conjugated

Unmodified

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

IHC: 1:100-1:200

IHC-P

Liquid

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human 15 Lipoxygenase 1