2. Immunology/Inflammation
  3. CD28
  4. Anti-Rat CD28 Antibody (JJ316)

Anti-Rat CD28 Antibody (JJ316) is a mouse-derived agonistic IgG1 κ type antibody, targeting to rat CD28. Anti-Rat CD28 Antibody (JJ316) can induce T cell proliferation. Anti-Rat CD28 Antibody (JJ316) can be used for the researches of inflammation, immunology and neurological disease, such as experimental autoimmune encephalomyelitis (EAE) and graft-versus-host disease (GvHD).

For research use only. We do not sell to patients.

Anti-Rat CD28 Antibody (JJ316)

Anti-Rat CD28 Antibody (JJ316) Chemical Structure

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Description

Anti-Rat CD28 Antibody (JJ316) is a mouse-derived agonistic IgG1 κ type antibody, targeting to rat CD28. Anti-Rat CD28 Antibody (JJ316) can induce T cell proliferation. Anti-Rat CD28 Antibody (JJ316) can be used for the researches of inflammation, immunology and neurological disease, such as experimental autoimmune encephalomyelitis (EAE) and graft-versus-host disease (GvHD)[1][2].

Isotype

Mouse IgG1 kappa
Species Reactivity

Rat
IC50 & Target

CD28
In Vivo

Anti-Rat CD28 Antibody (JJ316) (0.1 or 1 mg/rat, i.v., a single dose) CD4+CD25+ regulator T cell (Treg) from Lewis rats suppress myelin basic protein-driven T cell proliferation.[1].
Anti-Rat CD28 Antibody (JJ316) (0.1-1 mg/rat, i.v., a single dose) induces regulator T cell (Treg) proliferation in Adult Lewis rats[1].
Anti-Rat CD28 Antibody (JJ316) (0.03-1 mg/rat, i.v., a single dose) Reduced the severity of EAE in myelin oligodendrocyte glycoprotein (MOG)-induced experimental autoimmune encephalomyelitis (EAE) rat models[1].
Anti-Rat CD28 Antibody (JJ316) (1 mg/rat, i.v., a single dose) increase CD4+CD25+ T cells and increases IL-2, IL-4, IL-10 and Foxp3 levels in Lewis rats[2].
Anti-Rat CD28 Antibody (JJ316) (1 mg/rat, i.v., a single dose) suppresses graft-versus-host disease (GvHD) reactions in spleen transferred Lewis × DA hybrid rats[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Adult Lewis rats[1]
Dosage: 0.1, 0.3 and 1 mg/rat
Administration: Intravenously injection
Result: Led to a significant increase in CD25+ cells among CD4+ cells in peripheral lymph nodes and in the spleen.
Led to a increase in CD25+ cells among CD4- cells at 1 mg/rat.
Increased transferred Treg cells further up-regulated CD25 expression.
Showed 97% of Tconv cells undergone at least one cell division at 1 mg/rat.
Showed that the vast majority of CD4+CD25+ cells, and all CD4+CD25high cells, continued to express FoxP3.
Animal Model: Myelin oligodendrocyte glycoprotein (MOG)-induced experimental autoimmune encephalomyelitis (EAE) rat models[1]
Dosage: 0.03, 0.1, 0.3 and 1 mg/rat
Administration: Intravenously injection, at days 0 and 10
Result: Immediately ameliorated disease activity at 1 mg/rat.
Reduced the severity of EAE.
Animal Model: Spleen transferred Lewis × DA hybrid rats[2]
Dosage: 1 mg/rat
Administration: Intravenously injection
Result: Showed no enlargement was found in the PLN on the left side.
Inhibited the GvHD mortality significantly.
Showed relatively few infiltrating mononu-clear cells and almost normal tissue architecture.
Gene ID

12487  [NCBI]

Accession
Application

ELISA, FACS, Functional assay, Research in vivo
Conjugated

Unconjugated
Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.
Molecular Weight

150 kDa

SMILES

[Anti-Rat CD28 Antibody (JJ316)]
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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Anti-Rat CD28 Antibody (JJ316) Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Anti-Rat CD28 Antibody (JJ316)CD28T cellsEAEGvHDFoxp3MOGInhibitorinhibitorinhibit

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